Sensor arrays for detecting analytes in fluids

• US 20030198573A1
• Filed: 01/27/2003
• Published: 10/23/2003
• Est. Priority Date: 01/25/2002
• Status: Abandoned Application

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Forood, Behrouz B., Lebl, Michal
• Application Number:
  US10/352,779
• Publication Number:
  US 20030198573A1
• Time in Patent Office: Days
• US Class Current: 422/82.08
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 2201/0221 Portable; cableless; compact; hand-held

Abstract

In accordance with the objects outlined above, the present invention provides sensor arrays for the detection of target analytes. The sensor arrays comprising a substrate comprising a surface comprising discrete sites, each discrete site comprising a solvatochromic dye and at least one micro-environment moiety (MEM), both of which are preferably covalent attached to the discrete site. In some aspects, the discrete sites comprise microspheres to which the dyes and MEMs are attached, again, preferably covalently. The dye(s) and MEM(s) at each site can be independently attached, or they can be co-attached using a linker.

Claims

1. A sensor array for the detection of target analytes comprising a substrate comprising a surface comprising discrete sites, each discrete site comprising:

a) a covalently attached solvatochromic dye; and

b) at least one different covalently attached micro-environment moiety (MEM).

2. A sensor array according to claim 1 wherein each discrete site comprises a microsphere.

3. A sensor array according to claim 2 wherein said microspheres are distributed in wells on a surface of said substrate.

4. A sensor array according to claim 1 wherein said solvatochromic dye is Nile Red.

5. A sensor array according to claim 1 wherein said dye and said MEM are attached at different locations at said site.

6. A sensor array according to claim 1 further comprising a linker covalently attached to each of said sites, wherein said linker comprises said dye and said MEM.

7. A sensor array according to claim 6 wherein said linker is an oligomer.

8. A sensor array according to claim 7 wherein said oligomer is an amino acid.

9. A sensor array according to claim 1 wherein at least one site comprises a plurality of MEMs.

10. A sensor array according to claim 1 wherein at least one site comprises a plurality of solvatochromic dyes.

11. A sensor array according to claim 10 wherein at least two sites comprise a plurality of MEMs, and said two sites comprises a different plurality.

12. A sensor array for the detection of target analytes comprising a substrate comprising:

a) a population of microspheres comprising a first and a second subpopulation, each member of each subpopulation comprising;

i) a covalently attached solvatochromic dye; and

ii) at least one covalently attached micro-environment moiety (MEM);

wherein the MEM on each subpopulation is different;

wherein said microspheres are distributed at discrete sites on a surface of said substrate.

13. A method of detecting a target analyte in a sample comprising:

a) contacting said sample to a sensor array comprising;

i) a substrate comprising a surface comprising discrete sites, each discrete site comprising;

1) a covalently attached solvatochromic dye; and

2) at least one different covalently attached micro-environment moiety (MEM); and

b) measuring the optical response of a plurality of discrete sites.

14. A method according to claim 13 further comprising comparing said response to the response of a reference sample.