Enzymatic fuel cell
First Claim
1. A fuel cell with an anode compartment and a cathode compartment comprising:
- in the anode compartment, an anode electrode and one or more dehydrogenase enzymes effective to transfer electrons from a C1 compound to electron carrier(s), and wherein or further comprising one of the following;
(i) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to the anode electrode, (ii) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to electron transfer mediator(s) selected to be effective to deliver electrons to the anode electrode, wherein the anode compartment further comprises the electron transfer mediator(s), (iii) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to a redox enzyme, the redox enzyme is selected to be effective to deliver electrons to second electron carrier(s), the second electron carrier(s) selected to be effective to deliver electrons to electron transfer mediator(s) selected to be effective to deliver the electrons to the anode electrode, wherein the anode compartment further comprises the redox enzyme, second electron carrier(s) and electron transfer mediator(s);
in the cathode compartment, a cathode electrode which, when a conductive pathway to the first electrode is formed, is effective to convey the electrons to an electron acceptor composition in the cathode compartment; and
a barrier separating the anode compartment from the cathode compartment but effective to convey protons from the anode compartment to the cathode compartment.
1 Assignment
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Accused Products
Abstract
In one embodiment, provided is a fuel cell with an anode compartment and a cathode compartment comprising: in the anode compartment, an anode electrode and one or more dehydrogenase enzymes effective to transfer electrons from a C1 compound comprising carbon, oxygen and hydrogen (optionally consisting of carbon, oxygen and hydrogen) to electron carrier(s), and wherein or further comprising one of the following:
(i) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to the anode electrode,
(ii) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to electron transfer mediator(s) selected to be effective to deliver electrons to the anode electrode, wherein the anode compartment further comprises the electron transfer mediator(s),
(iii) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to a redox enzyme, the redox enzyme is selected to be effective to deliver electrons to second electron carrier(s), the second electron carrier(s) selected to be effective to deliver electrons to electron transfer mediator(s) selected to be effective to deliver the electrons to the anode electrode, wherein the anode compartment further comprises the redox enzyme, second electron carrier(s) and electron transfer mediator(s);
in the cathode compartment, a cathode electrode which, when a conductive pathway to the first electrode is formed, is effective to convey the electrons to an electron acceptor composition in the cathode compartment; and a barrier separating the anode compartment from the cathode compartment but effective to convey protons from the anode compartment to the cathode compartment.
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Citations
37 Claims
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1. A fuel cell with an anode compartment and a cathode compartment comprising:
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in the anode compartment, an anode electrode and one or more dehydrogenase enzymes effective to transfer electrons from a C1 compound to electron carrier(s), and wherein or further comprising one of the following;
(i) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to the anode electrode, (ii) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to electron transfer mediator(s) selected to be effective to deliver electrons to the anode electrode, wherein the anode compartment further comprises the electron transfer mediator(s), (iii) the electron carrier(s) are selected to operate with the dehydrogenase enzymes and to be effective to deliver electrons to a redox enzyme, the redox enzyme is selected to be effective to deliver electrons to second electron carrier(s), the second electron carrier(s) selected to be effective to deliver electrons to electron transfer mediator(s) selected to be effective to deliver the electrons to the anode electrode, wherein the anode compartment further comprises the redox enzyme, second electron carrier(s) and electron transfer mediator(s);
in the cathode compartment, a cathode electrode which, when a conductive pathway to the first electrode is formed, is effective to convey the electrons to an electron acceptor composition in the cathode compartment; and
a barrier separating the anode compartment from the cathode compartment but effective to convey protons from the anode compartment to the cathode compartment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of producing electrical power comprising:
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in an anode compartment, enzymatically reducing electron carrier(s) with electrons from a C1 compound, the electron carrier(s) selected to operate with the dehydrogenase enzymes;
directly transferring the electrons from the electron carrier(s) to an anode electrode;
transferring electrical current via an electrical conduit under an electrical load to a cathode electrode; and
transferring the electrons from the cathode electrode to an electron acceptor composition.
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11. A method of producing electrical power comprising:
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in an anode compartment, enzymatically reducing electron carrier(s) with electrons from a C1 compound, the electron carrier(s) selected to operate with the dehydrogenase enzymes;
directly transferring the electrons from the electron carrier(s) to the electron transfer mediator(s);
transferring the electrons from the electron transfer mediator(s) to the anode electrode;
transferring electrical current via an electrical conduit under an electrical load to a cathode electrode; and
transferring the electrons from the cathode electrode to an electron acceptor composition.
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12. A method of producing electrical power comprising:
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in an anode compartment, enzymatically reducing electron carrier(s) with electrons from a C1 compound, the electron carrier(s) selected to operate with the dehydrogenase enzymes;
enzymatically transferring the electrons from the electron carrier(s) to second electron carrier(s);
directly transferring the electrons from the second electron carrier(s) to the electron transfer mediator(s);
transferring electrons from the electron transfer mediator(s) to the anode electrode;
transferring electrical current via an electrical conduit under an electrical load to a cathode electrode; and
transferring electrons from the cathode electrode to an electron acceptor composition.
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13. A fuel cell with an anode compartment and a cathode compartment comprising:
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in the anode compartment, an anode electrode and electron carrier(s);
in the anode compartment, one or more dehydrogenase enzymes effective to transfer electrons from a C1 compound to an electron carrier;
in the cathode compartment, a cathode electrode which, when a conductive pathway to the first electrode is formed, is effective to convey the electrons to an electron acceptor composition in the cathode compartment; and
a barrier separating the anode compartment from the cathode compartment but comprising a proton pumping polypeptide effective to transport protons from the anode compartment to the cathode compartment. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method of producing electrical power comprising:
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in an anode compartment, enzymatically reducing electron carrier(s) with electrons from a C1 compound, the electron carrier(s) selected to operate with the dehydrogenase enzymes;
enzymatically transferring the electrons from the electron carrier(s) to the redox enzyme;
transferring the electrons from the redox enzyme to electron transfer mediator(s);
transferring the electrons from the electron transfer mediator(s) to the anode electrode;
transferring electrical current via an electrical conduit under an electrical load to a cathode electrode; and
transferring the electrons from the cathode electrode to an electron acceptor composition.
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29. A method of forming a biocompatible membrane that incorporates a polypeptide associated with the biocompatible membrane comprising:
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contacting an aperture with a mixture of polypeptide, membrane-forming amphiphile and an amount of a solvent miscible with water and biomembrane phase effective to decrease viscosity sufficiently to facilitate biocompatible membrane formation; and
removing the solvent by evaporation.
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- 30. A biocompatible membrane comprising a membrane-like barrier formed of triblock copolymer that comprises cross-linked polymer formed across an aperture with beveled edges.
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32. A method of preserving the function of a polypeptide in the presence of non-aqueous solvents comprising:
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forming a solution of block copolymers in a solvent comprising at least one non-aqueous solvent, and subsequently adding polypeptide to the solution. - View Dependent Claims (33, 34, 35, 36)
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37. A fuel cell with an anode compartment and a cathode compartment comprising:
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in the anode compartment, an anode electrode and one or more dehydrogenase enzymes effective to transfer electrons from a C1 compound to an electron carrier, the anode compartment further comprising a liquid for supporting the dehydrogenase enzymes and adapted to maintain during operation of the fuel cell a pH of 8.0 or higher;
in the cathode compartment, hydrogen peroxide and a cathode electrode which, when a conductive pathway to the first electrode is formed, is effective to convey the electrons to an electron acceptor composition in the cathode compartment, the anode adapted to maintain during operation of the fuel cell a pH of 5.0 or lower; and
a barrier separating the anode compartment from the cathode but effective to convey protons from the anode compartment to the cathode compartment.
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Specification