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Mesoscale polynucleotide amplification analysis

  • US 20030199081A1
  • Filed: 04/30/2003
  • Published: 10/23/2003
  • Est. Priority Date: 05/01/1992
  • Status: Active Grant
First Claim
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1. A device for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction, the device comprising:

  • a solid substrate microfabricated to define;

    a sample inlet port; and

    a mesoscale flow system comprising;

    a sample flow channel extending from said inlet port; and

    a polynucleotide polymerization reaction chamber, in fluid communication with said flow channel, containing reagents for the polymerization reaction; and

    means for thermally regulating the contents of said chamber whereby the temperature is controlled to amplify said preselected polynucleotide.

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