Preparation of collagen

• US 20030203008A1
• Filed: 04/02/2003
• Published: 10/30/2003
• Est. Priority Date: 01/13/1997
• Status: Abandoned Application

• Current Assignee: Subramanian Gunasekaran
• Original Assignee: Subramanian Gunasekaran
• Inventors: Gunasekaran, Subramanian
• Application Number:
  US10/406,331
• Publication Number:
  US 20030203008A1
• Time in Patent Office: Days
• US Class Current: 424/442
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]

Abstract

The present invention relates to methods for preparing collagen, especially type I collagen. In particular, the present invention provides methods for the preparation of collagen suitable for biomedical and veterinary applications. The collagen prepared according to the present invention provides numerous desirable characteristics for applications such as implantation, transplantation, and grafting.

Claims

1. A composition comprising 0.1 to 35.0% by weight of purified, phosphorylated collagen type I, wherein said collagen is prepared by the sequential steps of a first papain digestion, reduction, a second papain digestion, and phosphorylation.

2. The composition of claim 1, wherein said collagen has been sterilized after phosphorylation.

3. The composition of claim 1, wherein said composition is in a form selected from the group consisting of an emulsion, a dispersion, a slurry, a dry fiber, a particulate powder, and a sheet.

4. The composition of claim 1, further comprising at least one additive selected from the group consisting of an emollient, a solvent, a preservative, a moisturizing agent, an emulsifier, a fragrance, an antioxidant, an ultraviolet light protection filter, a pigment, a metal oxide, an anti-inflammatory agent, a pearlizing agent, a keratolytic substance and an antimicrobial.

5. The composition of claim 1, further comprising 0.1 to 95% by weight of an emollient selected from the group consisting of mineral oil, petrolatum, capric triglyceraldehydes, caprylic triglyceraldehydes, cholesterol, lanolin, dimethicone, cyclomethicone, almond oil, jojoba oil, avocado oil, sesame oil, sunflower oil, coconut oil, and grapeseed oil.

6. The composition of claim 1, further comprising 0.1 to 90% by weight of a solvent selected from the group consisting of purified water, ethyl alcohol, propyl alcohol and acetone.

7. The composition of claim 1, further comprising a preservative selected from the group consisting of methyl paraben, propyl paraben, butyl paraben, quaternium-8, quaternium-14, quaternium-15, imidazolidinyl urea, diazolidinyl urea, citric acid and ethanol.

8. The composition of claim 1, further comprising a moisturizing agent selected from the group consisting of glycerin, glycerol, propylene glycol, sorbitol, hyaluronic acid, lecithin, urea, DNA, lactic acid, glycolic acid, pyrrolidone carboxylic acid, phospholipids, collagen, elastin, ceramide, and squalene.

9. The composition of claim 1, further comprising an emulsifier selected from the group consisting of a polysorbate, sodium lauryl sulphate, sodium laureth sulphate, cetyl alcohol and cetearyl alcohol.

10. The composition of claim 1, further comprising a fragrance selected from the group consisting of a cyclopentanone, a cyclopentanol, a cyclopentylidene, and a cyclopentyl substituent, and wherein the fragrance does not cause skin irritation.

11. The composition of claim 1, further comprising an antioxidant selected from the group consisting of ascorbic acid, butylated hydroxyanisole, butylated hydroxytoluene, ethylene diamine tetracetic acid, and tocopherol.

12. The composition of claim 1, further comprising an ultraviolet light protection filter selected from the group consisting of a sun protection factor in the range of 2-20, p-aminobenzoic acid, a p-aminobenzoic acid derivative, a salicyclate, a dibenzoylmethane, a cinnamate, and a benzophenone.

13. The composition of claim 1, further comprising a pigment selected from the group consisting of a natural pigment, a synthetic pigment, and combinations thereof.

14. The composition of claim 1, further comprising a metal oxide selected from the group consisting of zinc oxide and titanium oxide.

15. The composition of claim 1, further comprising an anti-inflammatory agent selected from the group consisting of zinc pyrithione, selenium sulfide, sulfur, salicylic acid, epsilon amino-caproic acid, cyclosporin, and a corticosteroid

16. The composition of claim 1, further comprising a pearlizing agent selected from the group consisting of a pearlizing wax, a phospholipid, and a tri-glyceride.

17. The composition of claim 1, further comprising a keratolytic substance selected from the group consisting of an enzymatic agent, an alpha hydoxy acid, a salicylic acid, and an abrasive agent.

18. The composition of claim 1, further comprising an antimicrobial selected from the group consisting of doxycycline, minocycline, tetracycline, chlorhexidine, ofloxacin, metronidazole, ciprofloxacin, sanguinarine, and sparfloxacin.

19. The composition of claim 1, wherein said composition has a pH in the range of 2 to 12.

20. The composition of claim 1, wherein said composition is in the form of a cell culture material selected from the group consisting of a cell culture plate coating for growth of adherent cells and microparticles for growth of suspension cells.