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Detection of a target in a sample

  • US 20030203394A1
  • Filed: 06/03/2003
  • Published: 10/30/2003
  • Est. Priority Date: 05/04/1998
  • Status: Abandoned Application
First Claim
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1. A method for detecting a target nucleic acid molecule in a sample, said method comprising:

  • providing a device for detecting the presence of a target nucleic acid molecule in a sample, comprising;

    two electrical conductors, including a first electrical conductor and a second electrical conductor, but where the electrical conductors are not in contact, and one or more sets of two oligonucleotide probes attached to the electrical conductors, where the oligonucleotide probes are positioned such that the probes cannot come into contact with one another and such that a target nucleic acid molecule, which has two sequences, a first sequence complementary to a first probe attached to the first electrical conductor and a second sequence complementary to a second probe attached to the second electrical conductor, can bind to both probes concurrently;

    contacting the probes with a sample which may have the target nucleic acid molecule under selective hybridization conditions to permit target nucleic acid molecules, if any, present in the sample to hybridize to both of the probes;

    forming a conductive coating over the oligonucleotide probes and any target nucleic acid molecules hybridized to the oligonucleotide probes; and

    determining if an electrical current can be carried between the probes, said electrical current between the probes indicating the presence of the target nucleic acid molecule in the sample which has sequences complementary to the probes.

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