Stabilized tetrazolium reagent compositions and methods for using the same
First Claim
1. A reagent composition comprising:
- a tetrazolium dye; and
an effective amount of a nitrite stabilizing agent.
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Abstract
Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e.g., a nitrite salt. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; an electron transfer agent; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).
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Citations
32 Claims
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1. A reagent composition comprising:
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a tetrazolium dye; and
an effective amount of a nitrite stabilizing agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 17)
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11. A reagent test strip comprising:
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a substrate; and
an analyte oxidizing signal producing system present on said substrate, wherein said analyte oxidizing signal producing system includes a water soluble tetrazolium salt and a nitrite stabilizing agent. - View Dependent Claims (12, 13, 14, 15, 16)
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18. An analyte detection or measurement system comprising:
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(a) a reagent test strip comprising;
(i) a substrate; and
(ii) an analyte oxidizing signal producing system present on said substrate, wherein said signal producing system includes a water soluble tetrazolium salt capable of accepting a hydride to produce a water soluble formazan and a nitrite stabilizing agent; and
(b) an automated instrument.
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19. A method for detecting the presence or determining the concentration of an analyte in a sample, said method comprising:
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(a) applying said physiological sample to a reagent test strip comprising;
(i) a substrate; and
(ii) an analyte oxidizing signal producing system present on said substrate, wherein said signal producing system includes a water soluble tetrazolium salt capable of producing a water soluble formazan product and a nitrite stabilizing agent to produce a spot comprising said formazan product on said substrate;
(b) detecting said spot; and
(c) relating said detected spot to the-presence or concentration of said analyte in said physiological sample. - View Dependent Claims (20, 21, 22, 23)
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24. A kit for use in determining the concentration of an analyte in a physiological sample, said kit comprising:
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(a) a reagent test strip comprising;
(i) a substrate; and
(ii) an analyte oxidizing signal producing system present on said substrate, wherein said signal producing system includes a water soluble tetrazolium salt capable of producing a water soluble formazan product and a nitrite stabilizing agent; and
(b) at least one of;
(i) a means for obtaining said physiological sample and (ii) an analyte standard. - View Dependent Claims (25, 26, 27)
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28. A method for stabilizing a tetrazolium dye in a reagent composition, said method comprising:
including in said reagent composition an effective amount of a nitrite stabilizing agent. - View Dependent Claims (29, 30, 31, 32)
Specification