Transfection method and uses related thereto
First Claim
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1. A method of introducing nucleic acid molecules into cells, said method comprising the steps of:
- (a) affixing a nucleic acid molecule-containing mixture onto the surface of an object selected from a sphere, fiber, rod, and tube, wherein said nucleic acid molecule-containing mixture comprises (i) nucleic acid molecules to be introduced into cells and (ii) a carrier; and
(b) contacting cells and said affixed nucleic acid molecules under appropriate conditions for entry of said nucleic acid molecules into said cells, whereby said nucleic acid molecules are introduced into said cells.
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Abstract
The invention features a method of introducing nucleic acid molecules into cells by (a) affixing a nucleic acid molecule-containing mixture onto the surface of a an object, wherein the nucleic acid molecule-containing mixture includes (i) nucleic acid molecules to be introduced into cells and (ii) a carrier protein; and (b) contacting cells and the affixed nucleic acid molecules under appropriate conditions for entry of the nucleic acid molecules into the cells, whereby the nucleic acid molecules are introduced into the cells.
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Citations
20 Claims
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1. A method of introducing nucleic acid molecules into cells, said method comprising the steps of:
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(a) affixing a nucleic acid molecule-containing mixture onto the surface of an object selected from a sphere, fiber, rod, and tube, wherein said nucleic acid molecule-containing mixture comprises (i) nucleic acid molecules to be introduced into cells and (ii) a carrier; and
(b) contacting cells and said affixed nucleic acid molecules under appropriate conditions for entry of said nucleic acid molecules into said cells, whereby said nucleic acid molecules are introduced into said cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A composition comprising (i) an object selected from a sphere, fiber, rod, and tube, (ii) nucleic acid molecules to be introduced into cells, and (ii) a carrier;
- wherein said nucleic acid molecules and said carrier are affixed to said object such that, under appropriate conditions for entry of said nucleic acid molecules into cells, said nucleic acid molecules but not said object enter into said cells.
- View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
Specification