Method for measuring substance and testing piece

• US 20030203503A1
• Filed: 03/11/2003
• Published: 10/30/2003
• Est. Priority Date: 10/30/1995
• Status: Active Grant

• Current Assignee: Atsuko Katayama, Takao Fukuoka
• Original Assignee: Atsuko Katayama, Takao Fukuoka
• Inventors: Fukuoka, Takao, Katayama, Atsuko
• Granted Patent: US 6,777,243 B2
• Time in Patent Office: Days
• US Class Current: 436/170
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple analysis is made possible, and easy handling is possible.

Claims

1. A method for measuring an analyte, comprising a step of measuring a detectable substance by using an aqueous reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the formation reaction of the detectable substance is carried out in the presence of the layered inorganic compound dispersed in the aqueous reaction system to increase a reaction rate of the formation reaction.

2. The method according to claim 1, wherein the layered inorganic compound is a 2:

1 type clay mineral.

3. The method according to claim 2, wherein the 2:

1 type clay mineral is a swelling layered clay mineral.

4. The method according to claim 3, wherein the swelling layered clay mineral is at least one member selected from the group consisting of bentonite, smectite, vermicullite and synthetic fluoro-mica.

5. The method according to claim 4, wherein the smectite is synthetic smectite.

6. The method according to claim 5, wherein the synthetic smectite is at least one member selected from the group consisting of hectorite and saponite.

7. The method according to claim 1, wherein the detectable substance is a dyestuff detectable by an optical method.

8. The method according to claim 1, wherein the detectable substance is a compound selected from the group consisting of amines, imines, polyenes, aromatic compounds, heterocyclic compounds and complexes between ions and cyclic ligands.

9. The method according to claim 1, wherein the formation reaction of the detectable substance is an oxidation-reduction reaction.

10. The method according to claim 1, wherein a starting substance or an intermediate of the formation reaction of the detectable substance is a cationic compound which can be adsorbed to the layered inorganic compound.

11. The method according to claim 10, wherein the starting substance or the reaction intermediate is a diazonium salt.

12. The method according to claim 10, wherein the starting substance or the reaction intermediate is a tetrazolium salt.

13. The method according to claim 1, wherein a buffer or a dried product thereof is contained in the reaction system.

14. The method according to claim 1, wherein the layered inorganic compound in the reaction system is added in the form of solution or dispersion of the layered inorganic compound to the reaction system.