Lipid-comprising drug delivery complexes and methods for their production

• US 20030203865A1
• Filed: 04/30/2002
• Published: 10/30/2003
• Est. Priority Date: 04/30/2001
• Status: Abandoned Application

• Current Assignee: Targeted Genetics Corporation (Innoviva, Inc.)
• Original Assignee: Targeted Genetics Corporation (Innoviva, Inc.)
• Inventors: Harvie, Pierrot, Paul, Ralph, Cudmore, Sally, OʼMahony, Daniel J.
• Application Number:
  US10/136,187
• Publication Number:
  US 20030203865A1
• Time in Patent Office: Days
• US Class Current: 514/44
• CPC Class Codes: A61K 47/58 obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: A61K 48/0025 wherein the non-active part clearly interacts with the delivered nucleic acid
• CPC Class Codes: A61K 9/1272 with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids with cholesterol as the only non-phosphatidyl surfactant A61K9/127; lipids as modifying agent A61K47/543
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 15/88 using microencapsulation, e.g. using amphiphile liposome vesicle
• CPC Class Codes: C12N 2810/40 Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
• CPC Class Codes: C12N 2810/405 Vectors comprising RGD peptide
• CPC Class Codes: C12N 2810/854 from hormones

Abstract

Novel stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described. The complexes of the invention comprise a drug, at least one lipid species, optionally at least one polycation, and at least one targeting factor. The at least one lipid species may comprise a pegylated lipid. The complexes of the invention may provoke lower levels of inflammatory cytokines such as tumor necrosis factor-α (TNF-α). The method described herein provides for the large scale production of lipid-comprising drug delivery systems useful for gene therapy and other applications.

Claims

1. A lipid-nucleic acid complex comprising a compacted nucleic acid, a polycation, a targeting factor, and a lipid, wherein:

a) the targeting factor increases cellular bioavailability of the nucleic acid by a means other than interaction with a specific outer cell surface membrane receptor;

b) the complex does not comprise a protamine or a salt thereof; and

c) the mean diameter of the complex is greater than about 100 nm and less than 400 nm.

2. The complex of claim 1, wherein the targeting factor is a membrane disruptive polymer.

3. The complex of claim 1, wherein the mean diameter of the complex is about 300 nm or less.

4. The complex of claim 1, wherein the mean diameter of the complex is about 200 nm or less.

5. The complex of claim 1, further comprising a shielding agent.

6. The complex of claim 5, wherein the shielding agent increases circulatory half life of the complex, reduces binding of serum components to the complex, or reduces complement opsonization of the complex.

7. The complex of claim 5, wherein the shielding agent comprises polyethylene glycol (PEG).

8. The complex of claim 5, wherein the shielding agent is PEG.

9. The complex of claim 5, wherein the shielding agent comprises a pegylated lipid.

10. The complex of claim 1, wherein the polycation is a synthetic polycation, a polycationic polypeptide or salt thereof.

11. The complex of claim 10, wherein the polycation is a synthetic polycation.

12. The complex of claim 11, wherein the synthetic polycation is selected from the group consisting of polycationic methacryloxy polymers, polycationic methacrylate polymers and polycationic poly(alkenylimines).

14. The complex of claim 11, wherein the synthetic polycation is selected from the group consisting of polyethyleneimine (PEI), poly(2-methacryloxyethyltrimethyl ammonium bromide) (PMOETMAB), and a co-polymer of dimethylamino methacrylate and methacrylic ester.

15. The complex of claim 1, wherein the targeting factor is a membrane disruptive synthetic polymer.

16. The complex of claim 1, wherein the targeting factor functions to increase cellular bioavailability by increasing transcription of the nucleic acid of the complex, by increasing uptake of the nucleic acid into the cell, by increasing uptake into a cellular compartment, by increasing exit of the nucleic acid from a cellular compartment, or by increasing transport of nucleic acid across a cell membrane.

17. The complex of claim 1, wherein the targeting factor is a membrane translocating peptide (MTLP).

19. The complex of claim 1, wherein the targeting factor comprises a nuclear localization sequence.

20. The complex of claim 19, wherein the nuclear localization sequence is SV 40 NLS.

21. The complex of claim 1, further comprising a co-lipid.

22. The complex of claim 1, wherein the targeting factor is conjugated to a PEG moiety.

23. The complex of any one of claims 1 to 22, wherein the lipid is a cationic lipid.

24. The complex of claim 23, wherein the cationic lipid is 1,2-bis(oleoyloxy)-3-trimethylammoniopropane (DOTAP).

25. The complex of claim 23, wherein the cationic lipid is DOTAP.

26. The complex of claim 23, wherein the co-lipid is selected from the group consisting of cholesterol, diphytanoyl phosphatidylethanolamine (DPHPE), dioleoyl phosphatidylethanolamine (DOPE), dioleoyl phosphatidylcholine (DOPC), dilauryl phosphatidylethanolamine (DLPE), 1,2-distearoyl-sn-glycero-3-phosphatidylethanolamine (DSPE), and dimyristoyl phosphatidylethanolamine (DPME).

51. The complex of claim 1, wherein the complex is shielded.

53. The complex of claim 51, further comprising a compound containing polyethylene glycol moieties.

55. The complex of claim 53, wherein the compound is a pegylated lipid.

65. A method of delivering a nucleic acid to a cell comprising contacting the cell with a complex according to any one of claims 1-22, 27-31, 33-43, or 46-56.

66. A method of delivering a nucleic acid to a cell comprising contacting the cell with a complex according to claim 23.

67. A method of delivering a nucleic acid to a cell comprising contacting the cell with a complex according to claim 24.

68. A method of delivering a nucleic acid to a cell comprising contacting the cell with a complex according to claim 25.

69. A method of delivering a nucleic acid to a cell comprising contacting the cell with a complex according to claim 26.

71. A method according to claim 65, wherein the delivery is in vivo to an individual.

72. A method according to claim 66, wherein the delivery is in vivo to an individual.

73. A method according to claim 67, wherein the delivery is in vivo to an individual.

74. A method according to claim 68, wherein the delivery is in vivo to an individual.

75. A method according to claim 69, wherein the delivery is in vivo to an individual.

77. A method according to claim 71 wherein the delivery is intravenous.

78. A method according to claim 72 wherein the delivery is intravenous.

79. A method according to claim 73 wherein the delivery is intravenous.

80. A method according to claim 74 wherein the delivery is intravenous.

81. A method according to claim 75 wherein the delivery is intravenous.

83. A method according to claim 71 wherein the individual is a human.

84. A method according to claim 72 wherein the individual is a human.

85. A method according to claim 73 wherein the individual is a human.

86. A method according to claim 74 wherein the individual is a human.

87. A method according to claim 75 wherein the individual is a human.

89. A method according to claim 77 wherein the individual is a human.

90. A method according to claim 78 wherein the individual is a human.

91. A method according to claim 79 wherein the individual is a human.

92. A method according to claim 80 wherein the individual is a human.

93. A method according to claim 81 wherein the individual is a human.

13. The complex of 12, wherein the polycationic methacrylate polymer is comprised of dimethylamino methacrylate.

18. The complex of 14, wherein the membrane translocating peptide is selected from the group consisting of H₂N-KKAAAVLLPVLLAAP-COOH (Elan094), H₂N-KKKAAAVLLPVLLAAP (ZElan094), H₂N-kkkaavllpvllaap (ZElan207), and H₂N-KKKAAAVLLPVLLAAPREDL (ZElan094R).

27. A lipid-nucleic acid complex comprising a compacted nucleic acid and at least one lipid species that is fusogenic, wherein:

a) the complex has an aqueous core; and

b) the mean diameter of the complex is greater than about 100 nm and less than 400 nm.

29. The complex of claim 27, wherein the mean diameter of the complex is greater than about 100 nm and less than 200 nm.

30. The complex of claim 27, wherein the mean diameter of the complex is determined by incubation in 50% serum in buffer for about 1 hour.

31. The complex of claim 27, wherein the complex has reduced binding to complement C3A and C5A.

32. The complex of any one of claims 27-31, wherein the fusogenic lipid is a cone forming lipid.

33. The complex of claim 27, wherein the cone forming lipid is dioleoyl phosphatidylethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-[phospho-L-serine] (DOPS), or N,N dioleyl-N,N-dimethyl-1,6-hexanediammonium chloride (TODMAC6).

34. The complex of claim 27, wherein the fusogenic lipid is pH sensitive.

35. The complex of claim 34, wherein the lipid is anionic at physiological pH, and fusogenicity is increased at about pH 5.5 to about pH 4.5 relative to physiological pH.

36. The complex of claim 35, wherein at about pH 4.5 the lipid is neutral or cationic.

37. The complex of claim 35, wherein the lipid is cholesteryl hemisuccinate (CHEMS) or 1,2-dioleoyl-sn-glycero-3-[phosphoethanolamine-N-dodecanoyl (NC₁₂-DOPE).

38. The complex of claim 27, wherein the lipid is neutral or cationic.

39. The complex of claim 27, wherein the polycation is selected from the group consisting of synthetic polycations, polycationic polypeptides, and salts thereof.

40. The complex of claim 39, wherein the polycation is a synthetic polycation.

41. The complex of claim 40, wherein the synthetic polycation is selected from the group consisting of polycationic methacryloxypolymers, polycationic methacrylate polymers and polycationic poly(alkenylimines).

42. The complex of claim 41, wherein the synthetic polycationic methacrylate polymer is a polymer comprising dimethylamino methacrylate.

43. The complex of claim 40 is a synthetic polycation selected from the group consisting of polyethyleneimine (PEI), poly(2-methacryloxyethyltrimethyl ammonium bromide) (PMOETMAB), and a co-polymer of dimethylamino methacrylate and methacrylic ester.

44. A complex according to any one of claims 39-43, wherein the complex further comprises at least one co-lipid.

45. The complex of claim 44, wherein the complex comprises 1,2-distearoyl-sn-glycero-3-phosphotidylethanolamine (DSPE).

46. The complex of claim 27, wherein the complex further comprises at least one targeting factor that increases cellular bioavailability of the nucleic acid.

47. The complex of claim 46, wherein the presence of the targeting factor results in an increase in transcription of the nucleic acid, an increase in the uptake of nucleic acid into the cell, an increase in the uptake of nucleic acid into a cellular compartment, an increase in an exit of the nucleic acid from a cellular compartment, or an increase in transport of the nucleic acid across a membrane.

48. The complex of claim 46, wherein the targeting factor is selected from the group consisting of folate, insulin, an Arg-Gly-Asp (RGD) peptide, luteinizing hormone releasing hormone (LHRH), a membrane translocating peptide (MTLP) and a compound comprising a nuclear localization sequence.

49. The complex of claim 46, wherein the targeting factor is selected from the group consisting of galactose-H₂N-KKAAAVLLPVLLAAP-COOH (Elan094), galactose-H₂N-KKKAAAVLLPVLLAAP (ZElan094), galactose-H₂N-kkkaavllpvllaap (ZElan2O7), and galactose-H₂N-KKKAAAVLLPVLLAAPREDL (ZElan094R).

50. The complex of claim 27, wherein the lipid undergoes a structural change between physiologic pH and pH about 4.5 resulting in increased fusogenicity.

52. The complex of claim 27, wherein the complex is shielded.

54. The complex of claim 52, further comprising a compound containing polyethylene glycol moieties.

56. The complex of claim 54, wherein the compound is a pegylated lipid.

61. A complex according to any one of claims 27-31, 33-43, or 46-56, prepared by the method of claim 57.

62. A complex according to claim 44 prepared by the method of claim 57.

63. A complex according to claim 45 prepared by the method of claim 57.

64. A complex according to any one of claims 46 to 49 prepared by the method of claim 58.

70. A method of delivering a nucleic acid to a cell comprising contacting the cell with a complex according to claim 44.

76. A method according to claim 70, wherein the delivery is in vivo to an individual.

82. A method according to claim 76 wherein the delivery is intravenous.

88. A method according to claim 76 wherein the individual is a human.

94. A method according to claim 82 wherein the individual is a human.

28. A lipid-nucleic acid complex comprising a compacted nucleic acid, a polycation, a targeting factor and at least one lipid species, wherein:

a) the at least one lipid species is an anionic lipid;

b) the complex has an aqueous core;

c) the complex comprises at least one fusogenic moiety;

d) the mean diameter of the complex is greater than about 100 nm and less than 400 nm; and

,

wherein the complex does not comprise protamine or a salt thereof.

57. A method for preparing a lipid-nucleic acid complex comprising a compacted nucleic acid and at least one lipid species that is fusogenic, comprising:

a) mixing an aqueous micelle mixture comprising a lipid and at least one lipophilic surfactant with a nucleic acid mixture comprising a nucleic acid, wherein the lipid has or assumes fusogenic characteristics, and wherein at least one of the mixtures contains a component that causes the nucleic acid to compact; and

b) after the mixing removing the lipophilic surfactant from mixture resulting from step a).

58. The method of claim 57, further comprising including at least one targeting agent in at least one of the mixtures of step a).

59. A lipid-nucleic acid complex prepared by the method of claim 57.

60. A lipid-nucleic acid complex prepared by the method of claim 58.