Electrically responsive promoter system
First Claim
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1. A therapeutic delivery system comprising an electrical pulse generator operably coupled with genetically engineered cells in a mammalian tissue, wherein said genetically engineered cells further comprise a target gene operably coupled to an electrically responsive promoter.
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Abstract
The present invention provides methods and systems for regulating delivery of therapeutic proteins and nucleic acids. Specifically, this involves using a genetically engineered electrically responsive promoter operably linked to a therapeutic gene sequence, wherein expression of said sequence is controlled by an electrical pulse generator
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Citations
44 Claims
- 1. A therapeutic delivery system comprising an electrical pulse generator operably coupled with genetically engineered cells in a mammalian tissue, wherein said genetically engineered cells further comprise a target gene operably coupled to an electrically responsive promoter.
- 27. An expression vector, comprising an electrical response enhancer element, a tissue specific promoter heterologous to the element, and a coding sequence, wherein said promoter is operably linked to said coding sequence and said element is effective to cause expression of said coding sequence.
- 34. An apparatus for testing cells comprising an upper plate electrode, a lower plate electrode, and a porous membrane which is positioned between said upper and lower plate electrodes during operation.
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39. A method of treating a patient comprising providing the patient with a electrical pulse generator operably coupled with genetically engineered cells in a patient tissue, wherein said genetically engineered cells further comprise a target gene operably coupled to an electrically responsive promoter.
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40. A method providing a patient with a electrical pulse generator operably coupled with genetically engineered cells in a patient tissue, wherein said genetically engineered cells further comprise a target gene operably coupled to an electrically.
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