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Methods for analysis of gene expression

  • US 20030204322A1
  • Filed: 06/18/2003
  • Published: 10/30/2003
  • Est. Priority Date: 01/28/2000
  • Status: Abandoned Application
First Claim
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1. A method for analyzing gene expression comprising:

  • a) obtaining a plurality of target sequences, wherein the plurality of target sequences comprises cDNA;

    b) multiplex amplifying said plurality of target sequences, wherein multiplex amplifying comprises combining the plurality of target sequences, a plurality of target-specific primers, and one or more universal primers, thereby producing a plurality of amplification products;

    c) separating one or more members of the plurality of amplification products;

    d) detecting one or more members of the plurality of amplification products, thereby generating a set of gene expression data;

    e) storing the set of gene expression data in a database; and

    f) performing a comparative analysis on the set of gene expression data, thereby analyzing the gene expression.

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