Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices
First Claim
Patent Images
1. A biosensor, comprising:
- a substrate member;
a layer containing a photo-reactive crosslinking agent applied to said substrate member;
a pattern of active receptive material areas defined on said substrate by attachment with said crosslinking agent, said receptive material comprising molecules specific for an analyte of interest;
a pattern of inactive blocking material areas defined on said substrate by attachment with said crosslinking agent, said inactive blocking material areas being interposed with said active receptive material areas and being non-reactive with the analyte of interest;
said active receptive material and inactive blocking material areas defined on said substrate by a masking process wherein a mask is placed over said substrate member prior to irradiating said substrate member with an energy source sufficient for activating said photo-reactive crosslinking agent such that areas exposed by said mask define one of said active receptive material and inactive blocking material areas, and said areas shielded by said mask define the other of said blocking material and receptive material areas; and
wherein when said biosensor is exposed to a medium containing said analyte of interest, the analyte binds to said receptive material in said active areas and subsequently facilitate diffraction of transmitted or reflected light in a diffraction pattern corresponding to said active receptive material areas.
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Abstract
A biosensor includes a substrate member with a pattern of active areas of receptive material and a pattern of blocking material layers. The receptive material and blocking material are attached to the substrate member with a photo-reactive crosslinking agent activated in a masking process. The receptive material is specific for an analyte of interest.
109 Citations
26 Claims
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1. A biosensor, comprising:
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a substrate member;
a layer containing a photo-reactive crosslinking agent applied to said substrate member;
a pattern of active receptive material areas defined on said substrate by attachment with said crosslinking agent, said receptive material comprising molecules specific for an analyte of interest;
a pattern of inactive blocking material areas defined on said substrate by attachment with said crosslinking agent, said inactive blocking material areas being interposed with said active receptive material areas and being non-reactive with the analyte of interest;
said active receptive material and inactive blocking material areas defined on said substrate by a masking process wherein a mask is placed over said substrate member prior to irradiating said substrate member with an energy source sufficient for activating said photo-reactive crosslinking agent such that areas exposed by said mask define one of said active receptive material and inactive blocking material areas, and said areas shielded by said mask define the other of said blocking material and receptive material areas; and
wherein when said biosensor is exposed to a medium containing said analyte of interest, the analyte binds to said receptive material in said active areas and subsequently facilitate diffraction of transmitted or reflected light in a diffraction pattern corresponding to said active receptive material areas. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of making a biosensor, comprising the steps of:
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applying a photo-reactive crosslinking agent to a surface of a substrate member;
forming one of a receptive material layer and a blocking material layer over the crosslinking agent;
placing a mask over the substrate member, the mask having a configuration so as to shield at least one underlying area of the substrate member while exposing at least one adjacent area, and irradiating the substrate member and mask combination with an energy source sufficient to activate the crosslinking agent in the areas exposed by the mask, the activated crosslinking agent crosslinking with the respective receptive material or blocking material in the exposed areas;
cleaning the unreacted receptive material or blocking material from the shielded areas of the substrate member after removal of the mask;
forming a layer of the respective other of the blocking material and receptive material over the surface of the substrate member, and irradiating the substrate member with the energy source so as to activate the remaining crosslinking agent in the previously shielded areas, the activated crosslinking agent crosslinking with the respective blocking material or receptive material; and
wherein a resulting pattern of active receptive material areas and blocking material areas are defined according to the pattern of shielded and exposed areas of the mask. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification