Method of making protein arrays
First Claim
1. A method of producing an array of proteins comprising, a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, b) expressing the nucleic acid molecules to produce proteins, c) immobilizing the proteins to the support.
1 Assignment
0 Petitions
Accused Products
Abstract
Disclosed are methods of producing immobilized arrays of proteins. Included are methods for producing high density arrays of nucleic acids, amplifying arrays, and replicating such arrays. The nucleic acid molecules present on the support, whether amplified or not, are then expressed to produce proteins which are immobilized to the nucleic acid upon production or can be can be immobilized directly to the support. Alternatively, proteins can be bound to the nucleic acid molecules to produce protein arrays of the present invention. Arrays produced by the disclosed methods may include both nucleic acids and proteins or the nucleic acids can be removed from the array leaving the proteins. The disclosed methods also include replication of protein arrays in which a subset of the proteins that are produced can be transferred to an additional support where they are then immobilized.
16 Citations
8 Claims
-
1. A method of producing an array of proteins comprising,
a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, b) expressing the nucleic acid molecules to produce proteins, c) immobilizing the proteins to the support.
-
2. A method of producing an array of proteins comprising,
a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, and amplifying in situ the nucleic acid molecules, b) expressing the nucleic acid molecules to produce proteins, c) immobilizing the proteins to the support.
-
3. A method of producing an array of proteins comprising,
a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, and amplifying in situ the nucleic acid molecules, b) expressing the nucleic acid molecules to produce proteins, c) transferring at least a subset of proteins produced in step b) to an additional support, and d) immobilizing the subset to the additional support.
-
4. A method of producing an array of proteins comprising,
a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, and amplifying in situ the nucleic acid molecules, b) transferring at least a subset of nucleic acid molecules produced by said amplifying to an additional support, c) immobilizing the subset to the additional support, d) expressing the subset to produce proteins, e) immobilizing the proteins to the additional support.
-
5. A method of producing an array of proteins comprising,
a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, and amplifying in situ the nucleic acid molecules, b) transferring at least a subset of nucleic acid molecules produced by said amplifying to an additional support, c) immobilizing the subset of nucleic acid molecules to the additional support, d) expressing the subset of nucleic acid molecules to produce proteins, e) transferring at least a subset of proteins produced in step d) to a subsequent support, and f) immobilizing the subset of proteins to the subsequent support.
-
8. A method of producing an array of proteins comprising,
a) providing a first nucleic acid array comprising nucleic acid molecules immobilized to a support, b) immobilizing proteins to the nucleic acid molecules.
Specification
-
- Resources
-
Current AssigneeGeorge M. Church
-
Original AssigneeGeorge M. Church
-
InventorsChurch, George M.
-
Application NumberUS09/767,764Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/6CPC Class CodesC12Q 1/6837 using probe arrays or probe...C12Q 2525/149 incorporating a coding sequ...G01N 33/547 with antigen or antibody at...
