Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo

• US 20030207448A1
• Filed: 05/06/2003
• Published: 11/06/2003
• Est. Priority Date: 05/06/2002
• Status: Abandoned Application

• Current Assignee: Gregory Henry Revera
• Original Assignee: Gregory Henry Revera
• Inventors: Revera, Gregory Henry
• Application Number:
  US10/430,966
• Publication Number:
  US 20030207448A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C12N 15/873 Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
• CPC Class Codes: C12N 2517/04 Cells produced using nuclear transfer

Abstract

Methods to create human stem cells without creating or destroying a human embryo have been developed. The invention includes a method of creating a human stem cell by obtaining a non-human stem cell and removing the nucleus. The nucleus of a human donor cell is transferred to the non-human stem cell.

Claims

1. A method of creating a human stem cell, comprising:

(a) transferring the nucleus of a human somatic donor cell into an enucleated non-human mammalian stem cell forming a nuclear transfer unit; and

(b) activating the nuclear transfer unit.

2. A method as claimed in claim 1, where the non-human mammalian stem cell is pluripotent.

3. A method as claimed in claim 1, where the non-human mammalian stem cell is multipotent.

4. A method as claimed in claim 1, further comprising:

(c) culturing the human stem cell to yield a human stem cell line.

5. A method of creating a human stem cell, comprising:

(a) enucleating a non-human mammalian stem cell; and

(b) transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.

6. A method as claimed in claim 5, where the non-human mammalian stem cell is pluripotent.

7. A method as claimed in claim 5, where the non-human mammalian stem cell is multipotent.

8. A method as claimed in claim 5, further comprising:

(c) culturing the human stem cell to yield a human stem cell line.

9. A method of creating a human stem cell, comprising:

(a) enucleating a non-human mammalian stem cell;

(b) transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell forming a nuclear transfer unit; and

(c) activating the nuclear transfer unit.

10. A method as claimed in claim 9, where the non-human mammalian stem cell is pluripotent.

11. A method as claimed in claim 9, where the non-human mammalian stem cell is multipotent.

12. A method as claimed in claim 9, further comprising:

(d) culturing the human stem cell to yield a human stem cell line.

13. A method of creating a human stem cell, comprising:

(a) enucleating a human oocyte;

(b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte creating a first nuclear transfer unit;

(c) activating the first nuclear transfer unit.

14. A method as claimed in claim 13 further comprising:

(d) culturing the first nuclear transfer unit to yield at least one non-human stem cell;

15. A method as claimed in claim 13 further comprising:

(d) culturing the human stem cell to yield a human stem cell line.

16. A method of creating a human stem cell;

comprising;

(a) enucleating a human oocyte;

(b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte creating a first nuclear transfer unit;

(c) activating the first nuclear transfer unit;

(d) culturing the first nuclear transfer unit to yield at least one non-human stem cell;

(e) enucleating the non-human stem cell; and

(f) transferring the nucleus of a human somatic donor cell into the enucleated non-human stem cell to create a second nuclear transfer unit;

17. A method as claimed in claim 16 where the non-human stem cell is pluripotent.

18. A method as claimed in claim 16 where the non-human stem cell is multipotent.

19. A method as claimed in claim 16 further comprising:

(g) culturing the human stem cell to yield a human stem cell line.

20. A method of creating a human stem cell, comprising:

(a) enucleating a human oocyte;

(b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte creating a first nuclear transfer unit;

(c) activating the first nuclear transfer unit;

(d) culturing the first nuclear transfer unit to yield at least one non-human stem cell;

(e) enucleating the non-human stem cell;

(f) transferring the nucleus of a human somatic donor cell into the enucleated non-human stem cell to create a second nuclear transfer unit; and

(g) activating the second nuclear transfer unit.

21. A method as claimed in claim 20 where the non-human stem cell is pluripotent.

22. A method as claimed in claim 20 where the non-human stem cell is multipotent.

23. A method as claimed in claim 20 further comprising:

(h) culturing the human stem cell to yield a human stem cell line.

24. A method of creating a human stem cell, comprising:

(a) step for transferring the nucleus of a human somatic donor cell into an enucleated non-human mammalian stem cell forming a nuclear transfer unit; and

(b) step for activating the nuclear transfer unit.

25. A method of creating a human stem cell, comprising:

(a) step for enucleating a non-human mammalian stem cell; and

(b) step for transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.

26. A method of creating a human stem cell, comprising:

(a) step for enucleating a human oocyte;

(b) step for transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte.

27. Method for creating a nuclear transfer unit comprising:

(a) enucleating a non-human mammalian stem cell; and

(b) transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.

28. Method for creating a nuclear transfer unit comprising:

(a) step for enucleating a non-human mammalian stem cell; and

(b) step for transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.

29. Method for creating a nuclear transfer unit comprising:

(a) enucleating a human oocyte; and

(b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated oocyte.

30. Method for creating a nuclear transfer unit comprising:

(a) step for enucleating a human oocyte; and

(b) step for transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated oocyte.