Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
First Claim
1. A method of reprogramming a human cell, comprising culturing the cell in the presence of primate pluripotent stem (pPS) cells, embryoid body cells, or a cell lysate or conditioned medium prepared from such cells.
1 Assignment
0 Petitions
Accused Products
Abstract
Described in this disclosure is a new process whereby cells of one tissue type can be reprogrammed to produce cells of a different tissue type. Cells from a human donor are reprogrammed by culturing adjacent to primate pluripotent stem cells (in an undifferentiated or newly differentiated state) or in an environment supplemented by components taken from pPS cells. Simultaneously or in a subsequent step, the donor cells can be treated in a manner that enhances differentiation towards a different tissue type. In this manner, patients in need of tissue regeneration can be treated with cells differentiated and reprogrammed from their own autologous cell donation.
102 Citations
25 Claims
- 1. A method of reprogramming a human cell, comprising culturing the cell in the presence of primate pluripotent stem (pPS) cells, embryoid body cells, or a cell lysate or conditioned medium prepared from such cells.
-
21. A method for evaluating the effectiveness of a cell population for reprogramming a cell in a coculture system, comprising coculturing the cell to be reprogrammed with the cell population, simultaneously or sequentially culturing the cell in a growth environment that promotes differentiation of the cell or its progeny into a new lineage that is different from the lineage of the cell before coculturing with the cell population, and evaluating the effectiveness of the cell population based on whether the ability of the cell to produce progeny of the new lineage has been increased.
Specification