Compositions and methods for gene therapy
First Claim
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1. A composition suitable for administration to a mammal which comprises:
- (a) a carrier of polymeric material;
(b) a bioactive therapeutic factor; and
(c) a transfection agent.
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Abstract
The present invention relates to injectable compositions comprising biocompatible, swellable, substantially hydrophilic, non-toxic and substantially spherical polymeric material carriers which are capable of efficiently delivering bioactive therapeutic factor(s) physically linked to a transfection agent for use in embolization gene therapy. The present invention further relates to methods of embolization gene therapy, particularly for the treatment of angiogenic and non-angiogenic-dependent diseases, using the injectable compositions.
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Citations
78 Claims
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1. A composition suitable for administration to a mammal which comprises:
- (a) a carrier of polymeric material;
(b) a bioactive therapeutic factor; and
(c) a transfection agent. - View Dependent Claims (2, 3, 4, 60, 73)
- (a) a carrier of polymeric material;
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5. A composition suitable for active embolization which comprises:
- (a) a cross-linked substantially hydrophilic polymeric material suitable for embolization;
(b) a bioactive therapeutic factor; and
(c) a transfection agent. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 61, 67, 74)
- (a) a cross-linked substantially hydrophilic polymeric material suitable for embolization;
- 42. A method for delivering to a mammalian host a polynucleotide which comprises administering to a mammal having a disease a substantially hydrophilic polymeric material associated with a polynucleotide and a transfection agent.
- 54. A method for active embolization in a mammal host which comprises administering to a mammal having an angiogenesis dependent disease a substantially hydrophilic polymeric material associated with a bioactive therapeutic factor capable of expression of an anti-angiogenic material, wherein said bioactive therapeutic factor is associated with a transfection agent.
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62. A kit for performing embolization gene therapy comprising:
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(a) a suspension of microspheres suitable for embolization; and
(b) a transfection agent suitable for delivering genetic material to a cell. - View Dependent Claims (63, 64, 65, 66)
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- 68. A method for active embolization in a mammal host which comprises administering to a mammal having a disease a substantially hydrophilic polymeric material associated with a bioactive therapeutic factor, wherein said bioactive therapeutic factor is associated with a transfection agent.
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69. A microparticle suitable for active embolization which comprises a polymeric material capable of embolizing a blood vessel, wherein said polymeric material is linked to a transfection agent which is linked to a genetic material.
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77. A method for delivering to a mammalian host a polynucleotide which comprises administering to a mammal having a disease a substantially hydrophilic polymeric material associated with a polynucleotide and a transfection agent, and wherein said polymeric material is delivered to the site of action by the use of targeting antibodies.
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78. A method for active embolization in a mammal host which comprises administering to a mammal having an angiogenesis dependent disease a substantially hydrophilic polymeric material associated with a bioactive therapeutic factor capable of expression of an anti-angiogenic material, wherein said bioactive therapeutic factor is associated with a transfection agent, and wherein said polymeric material is delivered to the site of action by the use of targeting antibodies.
Specification