Method and apparatus for inactivation of biological contaminants using photosensitizers

• US 20030215784A1
• Filed: 02/28/2003
• Published: 11/20/2003
• Est. Priority Date: 07/21/1998
• Status: Abandoned Application

• Current Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Original Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Inventors: Goodrich, Raymond Paul, Dumont, Larry Joe
• Application Number:
  US10/377,524
• Publication Number:
  US 20030215784A1
• Time in Patent Office: Days
• US Class Current: 435/2
• CPC Class Codes: A61K 41/17 by ultraviolet [UV] or infrared [IR] light, X-rays or gamma rays
• CPC Class Codes: A61L 2/0076 using a photocatalyst or photosensitiser
• CPC Class Codes: A61L 2/0082 using chemical substances
• CPC Class Codes: A61L 2/0088 Liquid substances
• CPC Class Codes: A61M 1/0213 with isolated sections of the tube used as additive reservoirs
• CPC Class Codes: A61M 1/36222 Details related to the interface between cassette and machine
• CPC Class Codes: A61M 1/36224 with sensing means or components thereof
• CPC Class Codes: A61M 1/36226 Constructional details of cassettes, e.g. specific details on material or shape
• CPC Class Codes: A61M 1/362266 Means for adding solutions or substances to the blood
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3683 using photoactive agents
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: A61M 2205/123 with incorporated reservoirs
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Abstract

Methods and apparatuses are provided for inactivation of microorganisms in fluids or on surfaces. Preferably the fluids contain blood or blood products and comprise biologically active proteins. Preferred methods include the steps of adding an effective, non-toxic amount of an endogenous photosensitizer to a fluid and exposing the fluid to photoradiation sufficient to activate the endogenous photosensitizer whereby microorganisms are inactivated. Other fluids, including juices, water and the like, may also be decontaminated by these methods as may surfaces of foods, animal carcasses, wounds, food preparation surfaces and bathing and washing vessel surfaces. Alloxazines and K- and L-vitamins are among the preferred photosensitizers. Systems and apparatuses for flow-through and batch processes are also provided for decontamination of such fluids using photosensitizers.

Claims

1. A fluid comprising

a collected blood or blood component collected from a donor/patient;

and

an aqueous blood component additive solution comprising an endogenous photosensitizer selected from the group consisting of endogenous alloxazines, K vitamins and vitamin L.

2. The fluid of claim 1 wherein the endogenous photosensitizer is 7,8-dimethyl-10-ribityl isoalloxazine.

3. The fluid of claim 2 wherein the collected blood or blood component comprises platelets and the solution is a platelet additive solution further comprising:

physiological saline solution; and

tri-sodium citrate.

4. The fluid of claim 2 wherein the collected blood or blood component comprises platelets and the solution is a platelet additive solution further comprising:

sodium chloride; and

tri-sodium citrate.

5. The fluid of claim 3 further comprising:

sodium acetate.

6. The fluid of claim 5 further comprising:

potassium chloride;

magnesium chloride; and

sodium gluconate.

7. The fluid of claim 5 further comprising:

sodium phosphate.

8. The fluid of claim 7 further comprising:

potassium chloride;

magnesium chloride;

glucose; and

maltose.

9. The fluid of claim 4 further comprising:

potassium chloride; and

sodium phosphate.

10. The fluid of claim 9 further comprising:

calcium chloride;

magnesium sulfate;

citric acid;

sodium bicarbonate; and

glucose.

11. The fluid of claim 2 wherein the collected blood or blood component comprises red blood cells and the solution is an anticoagulant solution for red blood cells further comprising:

sodium citrate;

citric acid; and

dextrose.

12. The fluid solution of claim 11 further comprising:

monobasic sodium phosphate.

13. The fluid solution of claim 12 further comprising:

adenine.

14. The fluid of claim 2 wherein the collected blood or blood component comprises red blood cells and the solution is a red blood cell additive solution further comprising:

adenine

a nutrient selected from the group consisting of glucose and dextrose; and

sodium chloride.

15. The fluid of claim 14 further comprising:

mannitol.

16. The fluid of claim 14 further comprising:

monobasic sodium phosphate

citric acid; and

sodium citrate.

17. The fluid of claim 15 further comprising:

monobasic sodium phosphate; and

sodium citrate.

18. The fluid of claim 1 wherein the solution comprises a dry medium mixed with a solvent and the dry medium comprises the endogenous photosensitizer.

19. The fluid of claim 18 wherein the endogenous photosensitizer is 7, 8-dimethyl-10-ribityl isoalloxazine.

20. The fluid of claim 18 wherein the dry medium further comprises:

tri-sodium citrate.

21. The fluid of claim 20 wherein the dry medium further comprises:

sodium acetate.

22. The fluid of claim 21 wherein the dry medium further comprises:

potassium chloride;

magnesium chloride; and

sodium gluconate.

23. The fluid of claim 21 wherein the dry medium further comprises:

sodium phosphate.

24. The fluid of claim 23 wherein the dry medium further comprises:

potassium chloride

magnesium chloride;

glucose; and

maltose.

25. The fluid of claim 20 wherein the dry medium further comprises:

potassium chloride; and

sodium phosphate.

26. The fluid of claim 25, wherein the dry medium further comprises:

calcium chloride

magnesium sulfate;

citric acid;

sodium bicarbonate; and

glucose.

27. The fluid of claim 19 wherein the blood component additive solution comprises an anticoagulant preservative for red blood cells and the dry medium further comprises:

sodium citrate;

citric acid; and

dextrose.

28. The fluid of claim 27 wherein the dry medium further comprises:

monobasic sodium phosphate.

29. The fluid of claim 28 wherein the dry medium further comprises:

adenine.

30. The fluid of claim 19 wherein the blood component additive solution comprises a red blood cell additive solution and the dry medium further comprises:

adenine;

a nutrient selected from the group consisting of glucose and dextrose; and

;

sodium chloride.

31. The fluid of claim 30 wherein the dry medium further comprises:

mannitol.

32. The fluid of claim 31 wherein the dry medium further comprises:

monobasic sodium phosphate;

citric acid; and

sodium citrate.

33. The fluid of claim 31 wherein the dry medium further comprises:

monobasic sodium phosphate; and

sodium citrate.

34. The fluid of claim 19 wherein the dry medium is in a tablet, pill or capsule form.

35. The fluid of claim 21 wherein the dry medium is in a tablet, pill or capsule form.

36. The fluid of claim 22 wherein the dry medium is in a tablet, pill or capsule form.

37. The fluid of claim 23 wherein the dry medium is in a tablet, pill or capsule form.

38. The fluid of claim 24 wherein the dry medium is in a tablet, pill or capsule form.

39. The fluid of claim 25 wherein the dry medium is in a tablet, pill or capsule form.

40. The fluid of claim 26 wherein the dry medium is in a tablet, pill or capsule form.

41. The fluid of claim 27 wherein the dry medium is in a tablet, pill or capsule form.

42. The fluid of claim 28 wherein the dry medium is in a tablet, pill or capsule form.

43. The fluid of claim 29 wherein the dry medium is in a tablet, pill or capsule form.

44. The fluid of claim 30 wherein the dry medium is in a tablet, pill or capsule form.

45. The fluid of claim 31 wherein the dry medium is in a tablet, pill or capsule form.

46. The fluid of claim 32 wherein the dry medium is in a tablet, pill or capsule form.

47. The fluid of claim 33 wherein the dry medium is in a tablet, pill or capsule form.

48. The fluid of claim 19 wherein the solvent is saline.

49. The fluid of claim 34 further comprising a binder in the tablet.

50. The blood component additive solution of claim 2 further comprising a nutrient.

51. The blood component additive solution of claim 2 further comprising an enhancer.

52. The blood component additive solution of claim 50 further comprising an enhancer.

53. The blood component additive solution of claim 52 wherein the nutrient is glucose and the enhancer is a phosphate.

54. The fluid of claim 19 further comprising a nutrient.

55. The fluid of claim 19 further comprising an enhancer.

56. The fluid of claim 54 further comprising an enhancer.

57. The fluid of claim 56 wherein the nutrient is glucose and the enhancer is a phosphate.

58. The fluid of claim 50 wherein the nutrient is glucose.

59. The fluid of claim 50 wherein the nutrient is added to the fluid as a dry medium.

60. The fluid of claim 50 wherein the nutrient is added to the fluid as aqueous solution.

61. The fluid of claim 2 wherein the 7,8 dimethyl-10-ribityl isoalloxazine is added to the fluid as an aqueous solution.

62. The fluid of claim 2 wherein the 7,8 dimethyl-10-ribityl isoalloxazine is added to the fluid as a dry medium.

63. The fluid of claim 51 wherein the enhancer is added to the fluid as an aqueous solution.

64. The media of claim 51 wherein the enhancer is added to the fluid as a dry medium.

65. The fluid of claim 18 wherein the solvent comprises trisodium citrate and sodium acetate.

66. The fluid of claim 51 wherein the enhancer is a phosphate.