Detection of nucleic acid reactions on bead arrays
First Claim
1. A method of detecting a target nucleic acid sequence, said method comprising:
- a) attaching a first adapter nucleic acid to a first target nucleic acid sequence to form a modified first target nucleic acid sequence;
b) contacting said modified first target nucleic acid sequence with an array comprising;
i) a substrate with a patterned surface comprising discrete sites; and
ii) a population of microspheres comprising at least a first subpopulation comprising a first capture probe, such that said first capture probe and said modified first target nucleic acid sequence form a hybridization complex;
wherein said microspheres are distributed on said surface; and
c) detecting the presence of said modified first target nucleic acid sequence.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.
-
Citations
14 Claims
-
1. A method of detecting a target nucleic acid sequence, said method comprising:
-
a) attaching a first adapter nucleic acid to a first target nucleic acid sequence to form a modified first target nucleic acid sequence;
b) contacting said modified first target nucleic acid sequence with an array comprising;
i) a substrate with a patterned surface comprising discrete sites; and
ii) a population of microspheres comprising at least a first subpopulation comprising a first capture probe, such that said first capture probe and said modified first target nucleic acid sequence form a hybridization complex;
wherein said microspheres are distributed on said surface; and
c) detecting the presence of said modified first target nucleic acid sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
-
14. A method of detecting a target nucleic acid sequencingsequence comprising:
-
a) hybridizing a first primer to a first portion of a target sequence, wherein said first primer further comprises an adapter sequence;
b) hybridizing a second primer to a second portion of said target sequence;
c) ligating said first and second primers together to form a modified primer;
d) contacting said adapter sequence of said modified primer with an array comprising;
i) a substrate with a surface comprising discrete sites; and
ii) a population of microspheres comprising at least a first subpopulation comprising a first capture probe, such that said first capture probe and said modified primer form a hybridization complex;
wherein said microspheres are distributed on said surface; and
e) detecting the presence of said modified primer.
-
Specification