Devices, systems and methods for time domain multiplexing of reagents
First Claim
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1. A method of performing successive reactions in a microfluidic device, comprising:
- providing a microfluidic device comprising a reaction zone disposed within the microfluidic device, wherein the reaction zone is in fluid communication with a source of first reagent, a source of second reagent and a source of third reagent, the fluid connection between the second and third reagent sources and the reaction zone being configured to deliver the second reagent to the reaction zone prior to the third reagent;
applying a driving force to one of the reaction zone, the first reagent source, the second reagent source and the third reagent source to flow the first reagent through the reaction zone, introduce the second reagent into the reaction zone causing a first reaction between the first reagent and the second reagent, and subsequently introduce the third reagent into the reaction zone to cause a reaction between the first reagent and the third reagent.
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Abstract
Time dependent iterative reactions are carried out in microscale fluidic channels by configuring the channels such that reagents from different sources are delivered to a central reaction zone at different times during the analysis, allowing for the performance of a variety of time dependent, and/or iterative reactions in simplified microfluidic channels. Exemplary analyses include the determination of dose responses for biological and biochemical systems.
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Citations
26 Claims
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1. A method of performing successive reactions in a microfluidic device, comprising:
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providing a microfluidic device comprising a reaction zone disposed within the microfluidic device, wherein the reaction zone is in fluid communication with a source of first reagent, a source of second reagent and a source of third reagent, the fluid connection between the second and third reagent sources and the reaction zone being configured to deliver the second reagent to the reaction zone prior to the third reagent;
applying a driving force to one of the reaction zone, the first reagent source, the second reagent source and the third reagent source to flow the first reagent through the reaction zone, introduce the second reagent into the reaction zone causing a first reaction between the first reagent and the second reagent, and subsequently introduce the third reagent into the reaction zone to cause a reaction between the first reagent and the third reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of performing successive reactions in a microfluidic device, comprising:
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providing a microfluidic device comprising a reaction zone disposed within the microfluidic device, wherein the reaction zone is in fluid communication with a source of first reagent, a source of second reagent and a source of third reagent, the fluid connection between the second and third reagent sources and the reaction one being configured to deliver the second reagent to the reaction zone prior to the third reagent;
applying a driving force to one of the reaction zone, the first reagent source, the second reagent source and the third reagent source to flow the first reagent through the reaction zone, introduce the second reagent into the reaction zone causing a first reaction between the first reagent and the second reagent to produce a first product, and subsequently introduce the third reagent into the reaction zone to cause a reaction between the first product and the third reagent. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of determining a dose response of a first reagent on a biochemical system, comprising:
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providing a microfluidic device comprising a body structure, a reaction zone disposed within the body structure, the reaction zone being fluidly connected to a first reagent source, a second reagent source and a third reagent source, the first reagent source comprising a first reagent, the second reagent source comprising a second reagent at a first concentration and the third reagent source comprising the second reagent at a second concentration greater than the first concentration, wherein the fluid connection between the second reagent source and the reaction zone and the third reagent source and the reaction zone are configured to deliver the second concentration to the reaction zone subsequent to delivering the first concentration of the second reagent to the reaction zone;
detecting an effect of each of the first concentration of the second reagent and the second concentration of the second reagent on the first reagent within the reaction zone; and
generating a dose response curve from the detected effect. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26)
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Specification