Stochastic sensing through covalent interactions
First Claim
1. A system for sensing at least one reactive analyte in a solution comprising:
- a sensing device separated into a trans chamber and a cis chamber by a divider;
a protein pore operably disposed in the divider;
a detection system operable to detect current between the cis and trans chambers; and
an ionic solution containing at least one reactive analyte capable of covalently bonding to the protein pore;
wherein bonding of the reactive analyte to the protein pore produces a change in current between the cis and trans chambers detectable by the current detection system.
2 Assignments
0 Petitions
Accused Products
Abstract
A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered PSH type or αHL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.
-
Citations
53 Claims
-
1. A system for sensing at least one reactive analyte in a solution comprising:
-
a sensing device separated into a trans chamber and a cis chamber by a divider;
a protein pore operably disposed in the divider;
a detection system operable to detect current between the cis and trans chambers; and
an ionic solution containing at least one reactive analyte capable of covalently bonding to the protein pore;
wherein bonding of the reactive analyte to the protein pore produces a change in current between the cis and trans chambers detectable by the current detection system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
-
15. A system for sensing at least one reactive analyte in a sample comprising:
-
a sensor element; and
a sample containing at least a first reactive analyte, wherein the reactive analyte covalently bonds to the sensor element. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
-
-
31. A method for sensing at least one analyte in a solution comprising:
-
providing a sensing device separated into a trans chamber and a cis chamber by a divider, wherein a protein pore is operably disposed in the divider;
providing a detection system operable to detect current between the cis and trans chambers;
providing an ionic solution containing at least one reactive analyte capable of covalently bonding to the protein pore to the cis or trans chamber; and
detecting the current between the cis and trans chambers. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
-
-
43. A method for sensing at least one reactive analyte in a sample comprising:
-
providing a sensor element;
providing a sample containing at least a first reactive analyte capable of covalently bonding to the sensor element;
allowing the reactive analyte to covalently bond to the sensor element to produce a first signal; and
detecting the signal. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
-
Specification