Conductive microplate
First Claim
1. A conductive microplate for detecting target biomolecules in a sample, the microplate with a plurality of wells, the microplate comprising:
- an assembly of a porous substrate and a conductive layer, wherein the assembly is sealed into bottom of at least some wells, wherein the porous substrate has a top surface and a bottom surface, the top surface comprises a plurality of covalently attached probe biomolecules, wherein the probe biomolecules are reactive with target biomolecules, the conductive layer is attached to the bottom surface of the porous substrate, and the conductive layer is adapted to receive voltage.
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Abstract
A conductive microplate device for the detection of target biomolecules in a sample is described. The microplate comprises an assembly of a porous substrate and a conductive layer, wherein the assembly is sealed into bottom of at least some wells of the microplate. The porous substrate has a top surface and a bottom surface. The top surface comprises a plurality of covalently attached probe biomolecules. The covalently attached probe biomolecules are reactive with the target biomolecules contained in the sample. The conductive layer, which is attached to the bottom surface of the porous substrate, is adapted to receive voltage. Microplates of the present invention can be easily adapted for use with robotic workstations. Accordingly, in one embodiment, the power supply is incorporated into a robotic arm tool for fast microplate processing.
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Citations
33 Claims
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1. A conductive microplate for detecting target biomolecules in a sample, the microplate with a plurality of wells, the microplate comprising:
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an assembly of a porous substrate and a conductive layer, wherein the assembly is sealed into bottom of at least some wells, wherein the porous substrate has a top surface and a bottom surface, the top surface comprises a plurality of covalently attached probe biomolecules, wherein the probe biomolecules are reactive with target biomolecules, the conductive layer is attached to the bottom surface of the porous substrate, and the conductive layer is adapted to receive voltage. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 33)
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29. A method of forming a conductive microplate having a plurality of wells, the method comprising:
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(a) providing a porous substrate with a top surface and a bottom surface;
(b) activating the top surface with reactive functional groups capable of covalent attachment of probe biomolecules;
(c) contacting the top surface with probe biomolecules under conditions sufficient for covalent attachment of probe biomolecules to reactive functional groups;
(d) attaching a conductive layer to the bottom surface of the porous substrate; and
(e) sealing the porous substrate with the conductive layer into bottom of at least some wells of the microplate, wherein the conductive layer is adapted to receive voltage. - View Dependent Claims (30, 31, 32)
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Specification