Methods and compositions for organ decellularization
First Claim
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1. A solubilizing fluid for tissue decellularization comprising:
- an alkaline solution selected from the group consisting of sulphates, acetates, carbonates, bicarbonates, hydroxides, ammonium salts and combinations thereof; and
a non-ionic detergent wherein the concentration of detergent is in the range of about 0.006% (w/v) to 2.0% (w/v).
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Abstract
The invention is directed to methods for producing a decellularized organ or part of an organ. A decellularized organ is produced using an isolated organ mechanically agitated to remove cellular membranes surrounding the isolated organ without destroying the interstitial structure of the organ. After the cellular membrane is removed, the isolated organ is exposed to a solubilizing fluid that extracts cellular material without dissolving the interstitial structure of the organ. A washing fluid is used to remove the solubilized components, leaving behind a decellularized organ.
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3 Claims
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1. A solubilizing fluid for tissue decellularization comprising:
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an alkaline solution selected from the group consisting of sulphates, acetates, carbonates, bicarbonates, hydroxides, ammonium salts and combinations thereof; and
a non-ionic detergent wherein the concentration of detergent is in the range of about 0.006% (w/v) to 2.0% (w/v). - View Dependent Claims (2, 3)
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Specification