Method for preventing damage to or rejuvenating a cellular blood component using mitochondrial enhancer
First Claim
1. A method for treating a fluid comprising a cellular blood component to improve a vital quality of said cellular blood component, said method comprising adding an effective, substantially non-toxic amount of a mitochondrial enhancer to said fluid wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously-based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers.
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Abstract
This invention provides methods for treating cellular blood components and other cells containing mitochondria to improve vital qualities of the cells by contacting the cells with a mitochondrial enhancer to the cells. Mitochondrial enhancers prevent damage to and rejuvenate mitochondria and cells containing mitochondria. Mitochondrial enhancers include alloxazines and related compounds, such as riboflavin. Cells are optionally treated with photoradiation to reduce pathogens with may be present, before, after, and/or during treatment with mitochondrial enhancer. Treating with mitochondrial enhancer enables utilization of higher photoradiation energies, which achieves better pathogen reduction. When platelets are treated with mitochondrial enhancer, treated platelets may be stored for longer times than untreated platelets before they are administered to patients.
41 Citations
53 Claims
- 1. A method for treating a fluid comprising a cellular blood component to improve a vital quality of said cellular blood component, said method comprising adding an effective, substantially non-toxic amount of a mitochondrial enhancer to said fluid wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously-based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers.
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19. The method of claim I wherein the concentration of said 7,8-dimethyl-10-ribityl isoalloxazine in said fluid is about one to about 200 micromolar.
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36. A method for increasing the storage life of a cellular blood component comprising adding an effective, substantially non-toxic amount of a mitochondrial enhancer to said cellular blood component wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously-based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers.
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37. A method for extending platelet storage life comprising adding an effective, substantially non-toxic amount of a mitochondrial enhancer to said platelet wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously-based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers.
- 38. A method for treating a cell comprising a mitochondrion to improve a vital quality of said cell, comprising treating said cell with an effective, substantially non-toxic amount of a mitochondrial enhancer wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously-based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers.
- 40. A method for treating a fluid comprising a cell containing a mitochondrion to improve a vital quality of said fluid, comprising treating said cell with an effective, substantially non-toxic amount of a mitochondrial enhancer wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously-based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers.
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42. A method for treating a fluid comprising a cellular blood component to reduce pathogens which may be present therein, comprising the steps of:
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(a) adding an reduction-effective, substantially non-toxic amount of a photosensitizer to said fluid;
(b) adding an effective, substantially non-toxic amount of a mitochondrial enhancer different from said photosensitizer to said fluid;
(c) exposing said fluid to photoradiation of sufficient energy to activate said photosensitizer, for a sufficient time to substantially reduce said pathogens. wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers. - View Dependent Claims (43, 44, 45, 46, 47)
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48. A method for treating a fluid comprising a cellular blood component to reduce pathogens which may be present therein, comprising the steps of:
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(a) adding an reduction-effective, substantially non-toxic amount of a photosensitizer to said fluid;
(b) adding an effective, substantially non-toxic amount of a mitochondrial enhancer different from said photosensitizer to said fluid;
(c) exposing said fluid to photoradiation of sufficient energy to activate said photosensitizer, for a sufficient time to substantially reduce said pathogens. wherein said mitochondrial enhancer is selected from the group consisting of alloxazines, endogenous alloxazines, non-endogenous alloxazines, endogenously based derivative alloxazines, endogenous photosensitizers, and non-endogenous photosensitizers. - View Dependent Claims (49, 50, 51, 52, 53)
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Specification