Vascularization enhanced graft constructs
First Claim
1. An intestinal submucosa tissue graft construct for use in repairing diseased or damaged tissues, said tissue graft construct comprising vertebrate intestinal submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells.
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Abstract
An intestinal submucosa tissue graft construct for use in repairing diseased or damaged tissues is provided. The graft construct comprises vertebrate intestinal submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells which enhances initiation of the formation of vessel-like structures in the graft. The preselected population of cells can be a population of non-keratinized or keratinized epithelial cells or a population of mesodermally derived cells selected from the group consisting of fibroblasts, smooth muscle cells, skeletal muscle cells, cardiac muscle cells, multi-potential progenitor cells, pericytes, osteogenic cells, and any other suitable cell type, preferably selected based on the tissue to be repaired. Methods for enhancing the vascularization in vivo of these intestinal submucosa tissue graft constructs and for preparing these graft constructs are also provided.
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Citations
49 Claims
- 1. An intestinal submucosa tissue graft construct for use in repairing diseased or damaged tissues, said tissue graft construct comprising vertebrate intestinal submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells.
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11. A method for enhancing the vascularization in vivo of an intestinal submucosa tissue graft construct, said method comprising the steps of:
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seeding said intestinal submucosa tissue in vitro with a population of endothelial cells and at least one additional preselected, exogenous population of cells; and
implanting said graft construct into a vertebrate at a site in need of repair. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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- 33. A method of preparing an intestinal submucosa tissue graft construct for use in repairing diseased or damaged tissues, said method comprising the step of seeding said intestinal submucosa tissue in vitro with a population of endothelial cells, and at least one additional preselected, exogenous population of cells.
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35. A method for enhancing the vascularization in vivo of an intestinal submucosa tissue graft construct said method comprising the steps of:
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seeding said intestinal submucosa tissue in vitro with a population of endothelial cells and at least one additional preselected, exogenous population of cells;
culturing in vitro said endothelial cells on said intestinal submucosa tissue for a time sufficient to induce the formation of vessels or vessel-like structures; and
implanting said graft construct into a vertebrate in a site in need of repair. - View Dependent Claims (36, 37, 38, 39, 40, 41)
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42. A vascularized intestinal submucosa tissue graft construct for use in repairing diseased or damaged tissues, said tissue graft construct comprising vertebrate intestinal submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells wherein said endothelial cells have been cultured on said intestinal submucosa tissue for a time sufficient to form vessels or vessel-like structures in vitro.
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43. An intestinal submucosa tissue graft construct having vessel-like structures for use in repairing diseased or damaged tissues, said tissue graft construct comprising vertebrate intestinal submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells wherein said additional population of cells enhances the initiation of formation of said vessel-like structures in said graft construct.
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44. A method of preparing an intestinal submucosa tissue graft construct having vessel-like structures for use in repairing diseased or damaged tissues, said method comprising the step of seeding said intestinal submucosa tissue in vitro with a population of endothelial cells, and at least one additional preselected, exogenous population of cells wherein said additional population of cells enhances the initiation of formation of said vessel-like structures in said graft construct.
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45. A tissue graft construct for use in repairing diseased or damaged tissues, said tissue graft construct comprising submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells wherein the submucosa tissue is delaminated from the tunica muscularis layer and at least the luminal portion of the tunica mucosa layer of said tissue.
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46. A method of preparing a tissue graft construct for use in repairing diseased or damaged tissues, said method comprising the step of seeding in vitro submucosa tissue with a population of endothelial cells, and at least one additional preselected, exogenous population of cells wherein the submucosa tissue is delaminated from the tunica muscularis layer and at least the luminal portion of the tunica mucosa layer of said tissue.
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47. A tissue graft construct having vessel-like structures for use in repairing diseased or damaged tissues, said tissue graft construct comprising submucosa tissue, added endothelial cells, and at least one additional preselected, exogenous population of cells wherein said additional population of cells enhances the initiation of formation of said vessel-like structures in said graft construct.
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48. A method of preparing a tissue graft construct having vessel-like structures for use in repairing diseased or damaged tissues, said method comprising the step of seeding in vitro submucosa tissue with a population of endothelial cells, and at least one additional preselected, exogenous population of cells wherein said additional population of cells enhances the initiation of formation of said vessel-like structures in said graft construct.
Specification