Nucleic acid diagnostic reagents and methods for detecting nucleic acids, polynucleotides and oligonucleotides
First Claim
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1. A method comprising:
- (a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;
(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and activate the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;
(c) partitioning the active nucleic acid sequences from the rest of the candidate mixture;
(d) amplifying the active nucleic acid sequences to yield an enriched mixture of active nucleic acid sequences; and
(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction in the presence of the target nucleic acid molecule.
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Abstract
Methods for generating nucleic acid reagents useful for detecting nucleic acids, polynucleotides, and oligonucleotides are disclosed. Selection techniques, enzymatic nucleic acid molecules, allozymes (allosteric nucleic acid sensor molecules), ribozymes, and DNAzymes used as diagnostic reagents and tools are described.
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Citations
16 Claims
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1. A method comprising:
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(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;
(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and activate the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;
(c) partitioning the active nucleic acid sequences from the rest of the candidate mixture;
(d) amplifying the active nucleic acid sequences to yield an enriched mixture of active nucleic acid sequences; and
(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction in the presence of the target nucleic acid molecule. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12)
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2. In one embodiment, the invention features a method comprising:
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(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;
(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and inactivate the ability of the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;
(c) partitioning the inactive nucleic acid sequences from the rest of the candidate mixture;
(d) amplifying the inactive nucleic acid sequences to yield an enriched mixture of inactive nucleic acid sequences; and
(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction only in the absence of the target nucleic acid molecule.
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3. In another embodiment, the invention features a method comprising:
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(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;
(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and activate the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;
(c) partitioning the active nucleic acid sequences from the rest of the candidate mixture;
(d) amplifying the active nucleic acid sequences to yield an enriched mixture of active nucleic acid sequences;
(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction in the presence of the target nucleic acid molecule; and
(f) modifying the nucleic acid sensor molecule of (e), wherein the modification comprises adding, deleting, or substituting nucleotide residues and wherein such modification maintains the requirement of a target nucleic acid molecule for the catalytic activity of the nucleic acid sensor molecule. - View Dependent Claims (5)
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4. In another embodiment, the invention features a method comprising:
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(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;
(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and inactivate the enzymatic nucleic acid domain of such active member of the candidate mixture;
(c) partitioning the inactive nucleic acid sequences from the rest of the candidate mixture;
(d) amplifying the inactive nucleic acid sequences to yield an enriched mixture of inactive nucleic acid sequences;
(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction involving a reporter molecule in the absence of the target nucleic acid molecule; and
(f) modifying the nucleic acid sensor molecule of (e), wherein the modification comprises adding, deleting, or substituting nucleotide residues and wherein such modification maintains the catalytic activity of the nucleic acid sensor molecule in the absence of the target nucleic acid molecule.
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- 13. A nucleic acid sensor molecule, wherein said nucleic acid sensor molecule has sensor domain having a sequence of SEQ ID NOS:
Specification