Nucleic acid diagnostic reagents and methods for detecting nucleic acids, polynucleotides and oligonucleotides

• US 20030219775A1
• Filed: 12/16/2002
• Published: 11/27/2003
• Est. Priority Date: 12/14/2001
• Status: Abandoned Application

• Current Assignee: Yale University
• Original Assignee: Yale University
• Inventors: Ward, David C., Breaker, Ronald
• Application Number:
  US10/320,191
• Publication Number:
  US 20030219775A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6811 Selection methods for production or design of target specific oligonucleotides or binding molecules
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2521/337 Ribozyme
• CPC Class Codes: C12Q 2521/343 Abzyme
• CPC Class Codes: C12Q 2563/125 the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity

Abstract

Methods for generating nucleic acid reagents useful for detecting nucleic acids, polynucleotides, and oligonucleotides are disclosed. Selection techniques, enzymatic nucleic acid molecules, allozymes (allosteric nucleic acid sensor molecules), ribozymes, and DNAzymes used as diagnostic reagents and tools are described.

Claims

1. A method comprising:

(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;

(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and activate the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;

(c) partitioning the active nucleic acid sequences from the rest of the candidate mixture;

(d) amplifying the active nucleic acid sequences to yield an enriched mixture of active nucleic acid sequences; and

(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction in the presence of the target nucleic acid molecule.

6. The method of any of claims 1-4, wherein the sensor domain of (a) is from about 4 to about 100 nucleotides in length.

7. The method of any of claims 1-4, wherein the sensor domain of (a) is from about 8 to about 50 nucleotides in length.

8. The method of any of claims 1-4, wherein the sensor domain of (a) is from about 10 to about 20 nucleotides in length.

9. The method of any of claims 1-4, wherein the random sequence domain of (a) can be of equal or unequal length.

10. The method of any of claims 1-4, wherein the random sequence domain of (a) is from about 4 to about 200 nucleotides in length.

11. The method of any of claims 1-4, wherein the random sequence domain of (a) is from about 10 to about 50 nucleotides in length.

12. The method of any of claims 1-4, wherein the enzymatic nucleic acid domain of (a) comprises a hammerhead, inozyme, g-cleaver, hairpin, Zinzyme, Amberzyme, or DNAzyme.

2. In one embodiment, the invention features a method comprising:

(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;

(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and inactivate the ability of the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;

(c) partitioning the inactive nucleic acid sequences from the rest of the candidate mixture;

(d) amplifying the inactive nucleic acid sequences to yield an enriched mixture of inactive nucleic acid sequences; and

(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction only in the absence of the target nucleic acid molecule.

3. In another embodiment, the invention features a method comprising:

(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;

(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and activate the enzymatic nucleic acid domain of such active member of the candidate mixture to catalyze a reaction involving a reporter molecule;

(c) partitioning the active nucleic acid sequences from the rest of the candidate mixture;

(d) amplifying the active nucleic acid sequences to yield an enriched mixture of active nucleic acid sequences;

(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction in the presence of the target nucleic acid molecule; and

(f) modifying the nucleic acid sensor molecule of (e), wherein the modification comprises adding, deleting, or substituting nucleotide residues and wherein such modification maintains the requirement of a target nucleic acid molecule for the catalytic activity of the nucleic acid sensor molecule.

5. The method of claim 3 or claim 4, wherein the said nucleotide comprises a chemically modified nucleotide.

4. In another embodiment, the invention features a method comprising:

(a) generating a candidate mixture of nucleic acid sequences comprising a sensor domain having sequence complementary to a predetermined sequence in a target nucleic acid molecule where the sensor domain is flanked on either side by a random sequence domain and each of the random sequence domains is linked to a portion of an enzymatic nucleic acid domain;

(b) contacting the candidate mixture of nucleic acid sequences from (a) with the target nucleic acid molecule under conditions suitable for the target nucleic acid molecule to interact with the sensor domain of an active member of the candidate mixture and inactivate the enzymatic nucleic acid domain of such active member of the candidate mixture;

(c) partitioning the inactive nucleic acid sequences from the rest of the candidate mixture;

(d) amplifying the inactive nucleic acid sequences to yield an enriched mixture of inactive nucleic acid sequences;

(e) repeating steps (b)-(d), as necessary, to identify a nucleic acid sensor molecule capable of catalyzing a chemical reaction involving a reporter molecule in the absence of the target nucleic acid molecule; and

(f) modifying the nucleic acid sensor molecule of (e), wherein the modification comprises adding, deleting, or substituting nucleotide residues and wherein such modification maintains the catalytic activity of the nucleic acid sensor molecule in the absence of the target nucleic acid molecule.

13. A nucleic acid sensor molecule, wherein said nucleic acid sensor molecule has sensor domain having a sequence of SEQ ID NOS:

1-16.

14. The nucleic acid sensor molecule of claim 13, wherein one or more nucleotides of said nucleic acid sensor molecule comprises a nucleic acid backbone modification.

15. The nucleic acid sensor molecule of claim 13, wherein one or more nucleotides of said nucleic acid sensor molecule comprises a nucleic acid sugar modification.

16. The nucleic acid sensor molecule of claim 13, wherein one or more nucleotides of said nucleic acid sensor molecule comprises a nucleic acid base modification.