Novel glycoproteins and methods of use thereof
First Claim
1. An isolated nucleic acid molecule encoding a polypeptide comprising an amino acid sequence that is at least 75% identical to SEQ ID NO:
- 2 or 4, or the complement of said nucleic acid molecule.
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Abstract
The present invention provides novel isolated ARP/BRP polynucleotides and the membrane-associated or secreted polypeptides encoded by the ARP/BRP polynucleotides. Also provided are ARP and BRP protein multimers. Further provided are the antibodies that immunospecifically bind to a ARP/BRP polypeptide or any derivative, variant, mutant or fragment of the ARP/BRP polypeptide, a ARP/BRP multimer polynucleotide or antibody. The invention additionally provides methods in which the ARP/BRP polypeptide, multimer, polynucleotide and antibody are utilized in the detection and treatment of a broad range of pathological states, e.g. reproductive disorder, as well as to other uses.
23 Citations
40 Claims
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1. An isolated nucleic acid molecule encoding a polypeptide comprising an amino acid sequence that is at least 75% identical to SEQ ID NO:
- 2 or 4, or the complement of said nucleic acid molecule.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 10, 11, 27, 29, 36)
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8. An isolated nucleic acid molecule, wherein said nucleic acid molecule:
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(a) encodes a polypeptide having the amino acid sequence of SEQ ID NO;
2; and
(b) comprises contiguous nucleotides encoding the amino acid sequences. WEKPI (SEQ ID NO;
5);
or the complement of said nucleic acid molecule.
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9. A nucleic acid molecule less than 100 nucleotides in length and comprising at least 6 contiguous nucleotides of SEQ ID NO:
- 1 or a complement thereof.
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12. An isolated polypeptide at least 80% identical to a polypeptide selected from the group consisting of:
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a) a polypeptide comprising an amino acid sequence of SEQ ID NO;
2 or 4;
b) a fragment of a polypeptide comprising an amino acid sequence of SEQ ID NO;
2 or 4, wherein the fragment comprises at least 6 contiguous amino acids of SEQ ID NO;
2;
c) a derivative of a polypeptide comprising an amino acid sequence of SEQ ID NO;
2 or 4;
d) an analog of a polypeptide comprising an amino acid sequence of SEQ ID NO;
2 or 4;
e) a homolog of a polypeptide comprising an amino acid sequence of SEQ ID NO;
2 and 4; and
f) a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence of SEQ ID NO;
2 or 4, wherein the polypeptide is encoded by a nucleic acid molecule that hybridizes to a nucleic acid molecule of SEQ ID NO;
1 or 3, under stringent conditions. - View Dependent Claims (13, 14, 15, 16, 17, 19, 20, 22, 23, 24, 25, 26, 28, 30, 32, 34)
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- 18. A protein multimer comprising an ARP polypeptide and a second polypeptide.
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31. A method of treating or preventing a reproductive disorder in a subject, the method comprising administering to a subject method comprising administering to a subject in need thereof a therapeutic selected from the group consisting of:
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a) a ARP/BRP nucleic acid;
b) a ARP/BRP polypeptide and c) a ARP/BRP antibody;
wherein said therapeutic is administered in an amount sufficient to treat or prevent said reproductive disorder in said subject.
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33. A pharmaceutical composition comprising a therapeutically or prophylactically effective amount of a therapeutic selected from the group consisting of:
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a) a ARP/BRP nucleic acid;
b) a ARP/BRP polypeptide and c) a ARP/BRP antibody and a pharmaceutically acceptable carrier. - View Dependent Claims (35, 37)
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38. A method for determining the presence of or predisposition to a reproductive disorder in a subject, the method comprising:
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a) measuring the amount of a ARP/BRP polypeptide or ARP/BRP multimer in a sample from the subject; and
b) comparing the amount of said polypeptide in step (a) to the amount of the polypeptide present in a control sample, wherein an alteration in the level of the polypeptide or multimer in step (a) as compared to the control sample indicates a disease condition.
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39. A method for determining the presence of or predisposition to a reproductive disorder in a subject, the method comprising:
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a) measuring the amount of a ARP/BRP nucleic acid in a sample from the mammalian subject; and
b) comparing the amount of said nucleic acid in step (a) to the amount of the nucleic acid present in a control sample, wherein an alteration in the level of the nucleic acid in step (a) as compared to the control sample indicates a disease condition.
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40. A method for expressing an ARP/BRP polyppetide as a product if an endogenous gene in a cell, wherein the polypeptide is expressed at a modified level in a comparison to the wild type cell, the method comprising;
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(a) transfecting the cell with a DNA constuct, the DNA constrict comprising a transcription regulatory element in operative connection to the endogenous gene, thereby producing a recombinant cell and/or (b) transfecting the cell with a DNA constuct, the DNA constrict comprising a amplifiable gene and a DNA targeting sequence capable of inserting the amplifiable gene in operative connection to the endogenous gene, thereby producing a recombinant cell a (c) culturing the recombinant cell, and if desired, selecting cells containing multiple copies of the endogenous gene.
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Specification