Induction of insulin-producing cells

• US 20030219894A1
• Filed: 04/15/2003
• Published: 11/27/2003
• Est. Priority Date: 04/17/2002
• Status: Abandoned Application

• Current Assignee: JCR Pharmaceuticals Co., Ltd., Susumu Seino
• Original Assignee: JCR Pharmaceuticals Co., Ltd., Susumu Seino
• Inventors: Okuno, Masaaki, Ishizuka, Nobuko, Seino, Susumu
• Application Number:
  US10/413,358
• Publication Number:
  US 20030219894A1
• Time in Patent Office: Days
• US Class Current: 435/370
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2501/60 Transcription factors
• CPC Class Codes: C12N 2506/14 from hepatocytes
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0676 Pancreatic cells

Abstract

What is described is a method for preparation of insulin-producing cells from non-insulin-producing cells. Mammalian fetal hepatocytes or hepatic progenitor cells are used ad the non-insulin-producing cells, and the method comprises culturing the mammalian fetal hepatocytes or the hepatic progenitor cells with 1-50 mmol/L of nicotinamide and concurrently bringing about expression of the PDX-1 gene or the NeuroD gene in the mammalian fetal hepatocytes.

Claims

1. A method for preparation of insulin-producing cells from non-insulin-producing cells, wherein the non-insulin-producing cells are mammalian fetal hepatocytes, and wherein the method comprises culturing the mammalian fetal hepatocytes with 1-50 mmol/L of nicotinamide and concurrently bringing about expression of the PDX-1 gene or the NeuroD gene in the mammalian fetal hepatocytes.

2. The method of claim 1, wherein the expression of the gene is achieved through introduction of the gene into the mammalian fetal hepatocytes.

3. The method of claim 1 or 2, wherein the mammalian fetal hepatocytes are non-human, mammalian fetal hepatocytes.

10. Insulin-producing cells prepared by the method according to one of claims 1 to 9.

4. A method for preparation of insulin-producing cells from non-insulin-producing cells, wherein the non-insulin-producing cells are mammalian hepatic progenitor cells, and wherein the method comprises culturing the mammalian hepatic progenitor cells with 1-50 mmol/L of nicotinamide and concurrently bringing about expression of the PDX-1 gene or the NeuroD gene in the mammalian hepatic progenitor cells.

5. The method of claim 4, wherein the expression of the gene is achieved through introduction of the gene into the mammalian hepatic progenitor cells.

6. The method of claim 4 or 5, wherein the mammalian hepatic progenitor cells are non-human, mammalian hepatic progenitor cells.

7. A method for causing transdifferentiation of mammalian hepatic progenitor cells into insulin-producing cells, wherein the method comprises culturing the mammalian hepatic progenitor cells with 1-50 mmol/L of nicotinamide and bringing about expression of the PDX-1 gene or the NeuroD gene in the mammalian hepatic progenitor cells.

8. The method of claim 7, wherein the expression of the gene is achieved through introduction of the gene into the mammalian hepatic progenitor cells.

9. The method of claim 7 or 8, wherein the mammalian hepatic progenitor cells are non-human, mammalian hepatic progenitor cells.