Reverse screening and target identification with minicells
First Claim
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1. A method of identifying a nucleic acid that encodes a protein that binds to or chemically alters a preselected ligand, comprising:
- (a) separately contacting said ligand with individual members of a minicell library, wherein minicells in said library comprise expression elements, wherein said expression elements comprise DNA inserts, wherein an ORF in said DNA insert is operably linked to expression sequences, in order to generate a series of reaction mixes, each reaction mix comprising a different member of said minicell library;
(b) incubating said reaction mixes, thereby allowing a protein that binds to or chemically alters said preselected ligand to bind or chemically alter said preselected ligand;
(c) detecting a change in a signal from reaction mixes in which said ligand has been bound or chemically altered;
(d) preparing DNA from reaction mixes in which said ligand has been bound or chemically altered;
wherein said DNA is a nucleic acid that encodes a protein that binds to or chemically alters said preselected ligand.
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Abstract
The invention provides compositions and methods for the production of achromosomal and anucleate cells useful for applications such as diagnositic and therapeutic uses, as well as research tools and agents for drug discovery.
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Citations
20 Claims
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1. A method of identifying a nucleic acid that encodes a protein that binds to or chemically alters a preselected ligand, comprising:
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(a) separately contacting said ligand with individual members of a minicell library, wherein minicells in said library comprise expression elements, wherein said expression elements comprise DNA inserts, wherein an ORF in said DNA insert is operably linked to expression sequences, in order to generate a series of reaction mixes, each reaction mix comprising a different member of said minicell library;
(b) incubating said reaction mixes, thereby allowing a protein that binds to or chemically alters said preselected ligand to bind or chemically alter said preselected ligand;
(c) detecting a change in a signal from reaction mixes in which said ligand has been bound or chemically altered;
(d) preparing DNA from reaction mixes in which said ligand has been bound or chemically altered;
wherein said DNA is a nucleic acid that encodes a protein that binds to or chemically alters said preselected ligand. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of determining the amino acid sequence of a protein that binds or chemically alters a preselected ligand, comprising:
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(a) contacting said ligand with a minicell library, wherein minicells in said library comprise expression elements, wherein said expression elements comprise DNA inserts, wherein an ORF in said DNA insert is operably linked to expression sequences;
(b) incubating said mixture of ligand and minicells, under conditions which allow complexes comprising ligands and minicells to form and/or chemical reactions to occur;
(c) isolating or identifying said complexes from said ligand and said mixture of ligand and minicells;
(d) preparing DNA from an expression element found in one or more of said complexes, or in a minicell thereof;
(e) determining the nucleotide sequence of said ORF in said DNA;
(f) using the genetic code to predict an amino acid sequence encoded by said ORF, and (g) wherein said amino acid sequence is or is derived from a protein that binds or chemically alters a preselected ligand. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of identifying a nucleic acid that encodes a protein that inhibits or blocks an agent from binding to or chemically altering a preselected ligand, comprising:
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(a) separately contacting said ligand with individual members of a minicell library, wherein minicells in said library comprise expression elements, wherein said expression elements comprise DNA inserts, wherein an ORF in said DNA insert is operably linked to expression sequences, in order to generate a series of reaction mixes, each reaction mix comprising a different member of said minicell library;
(b) incubating said reaction mixes, thereby allowing a protein that binds to or chemically alters said preselected ligand to bind or chemically alter said preselected ligand;
(c) detecting a change in a signal from reaction mixes in which said ligand has been bound or chemically altered;
(d) preparing DNA from reaction mixes in which said change in signal ligand has been bound or chemically altered;
wherein said DNA is a nucleic acid that encodes a protein that inhibits or blocks said agent from binding to or chemically altering said preselected ligand - View Dependent Claims (18, 19, 20)
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Specification