Integrated cartridge for sample manipulation
First Claim
Patent Images
1. A device for automated sample manipulation, comprising:
- a liquid inlet well for providing a test sample;
a liquid input chamber for receiving said test sample from said liquid inlet well;
a first reaction chamber for performing a first reaction on said test sample;
a second reaction chamber for performing a second reaction on said test sample;
a first heating surface for heating said first and second reaction chambers; and
a denature chamber for denaturing said test sample.
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Abstract
An integrated cartridge for automated sample manipulation and, particularly strand displacement amplification, is provided. The cartridge comprises a sealed, two-part device with internal fluid channels and chambers, as well as reagents. The cartridge performs the sequence of fluid transfers, reagent additions and heat transitions, such as those of the strand displacement amplification process, in a single, sealed device.
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Citations
27 Claims
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1. A device for automated sample manipulation, comprising:
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a liquid inlet well for providing a test sample;
a liquid input chamber for receiving said test sample from said liquid inlet well;
a first reaction chamber for performing a first reaction on said test sample;
a second reaction chamber for performing a second reaction on said test sample;
a first heating surface for heating said first and second reaction chambers; and
a denature chamber for denaturing said test sample. - View Dependent Claims (2, 3)
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4. A device for automated DNA amplification, comprising:
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a liquid inlet well for providing a test sample containing DNA;
a liquid input chamber for receiving said test sample from said liquid inlet well;
a first reaction chamber for performing a prime reaction on the DNA in said test sample;
a second reaction chamber for performing an amplification reaction on the DNA in said test sample;
a first heating surface for heating said first and second reaction chambers; and
a denature chamber for denaturing the DNA in said test sample. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A device for automated sample manipulation, comprising a sealed cartridge having a cartridge top and a cartridge bottom, wherein said cartridge top comprises:
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a liquid inlet well;
fluidic cavities;
first, second and third connection ports;
first and second heat block surfaces; and
a take-out well. - View Dependent Claims (15, 16)
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17. A device for automated sample manipulation, comprising a sealed cartridge having internal fluidic cavities, wherein said internal fluidic cavities comprise, in fluid sequence:
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a liquid input chamber;
a sensing chamber;
a first reaction chamber;
a second reaction chamber;
a filter means;
a denature chamber; and
a take-out well. - View Dependent Claims (18, 19, 20)
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21. A method of sample manipulation, comprising:
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(a) placing a liquid sample into a liquid inlet well of a sealed cartridge;
(b) allowing the liquid sample to flow from the liquid inlet well to a liquid input chamber;
(c) moving the liquid sample from the liquid input chamber to a first reaction chamber;
(d) performing a first reaction on the liquid sample in the first reaction chamber;
(e) moving the liquid sample from the first reaction chamber to a second reaction chamber;
(f) performing a second reaction on the liquid sample in the second reaction chamber;
(g) moving the liquid sample from the second reaction chamber to a denature chamber; and
(h) denaturing the liquid sample. - View Dependent Claims (22, 23)
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24. A method of automated DNA amplification, comprising:
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(a) placing a liquid sample containing DNA into a liquid inlet well of a sealed cartridge;
(b) allowing the liquid sample to flow from the liquid inlet well to a liquid input chamber;
(c) moving the liquid sample from the liquid input chamber to a first reaction chamber;
(d) performing a first, prime reaction on the DNA in the liquid sample in the first reaction chamber;
(e) moving the liquid sample from the first reaction chamber to a second reaction chamber;
(f) performing a second, amplification reaction on the DNA in the liquid sample in the second reaction chamber;
(g) moving the liquid sample from the second reaction chamber to a denature chamber; and
(h) denaturing the DNA in the liquid sample. - View Dependent Claims (25, 26)
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27. A process of making a sealed cartridge for automated DNA amplification, comprising:
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(a) providing a cartridge top, wherein said cartridge top comprises a liquid inlet well;
fluidic cavities including a first reaction chamber and a second reaction chamber;
first, second and third connection ports;
first and second heat block surfaces; and
a take-out well;
(b) drying reagents in the first and second reaction chambers;
(c) providing a cartridge bottom;
(d) placing an adhesive pattern onto the cartridge bottom; and
(e) bonding the cartridge top and the cartridge bottom together to form the sealed cartridge.
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Specification