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Detection of abused substances and their metabolites using nucleic acid sensor molecules

  • US 20030224435A1
  • Filed: 05/16/2003
  • Published: 12/04/2003
  • Est. Priority Date: 05/16/2002
  • Status: Abandoned Application
First Claim
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1. A method of detecting the presence of a compound in a biological sample, the method comprising:

  • (a) contacting the sample with an nucleic acid sensor molecule, and (b) assaying for the presence of the drug under conditions suitable for detecting the presence of the drug in the sample, wherein the nucleic acid sensor molecule comprises a enzymatic nucleic acid component and one or more sensor components that upon interaction with the compound induces a chemical reaction of the enzymatic nucleic acid component that modulates the activity or properties of the reporter molecule, signaling the presence of the compound.

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