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Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent for environmental testing thereby

  • US 20030225529A1
  • Filed: 12/18/2002
  • Published: 12/04/2003
  • Est. Priority Date: 06/26/2001
  • Status: Abandoned Application
First Claim
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1. A method of identifying an unknown bioagent in an environmental sample using a database of molecular masses of known bioagents comprising:

  • contacting nucleic acid from said bioagent in said environmental sample with at least one pair of oligonucleotide primers that hybridize to sequences of said nucleic acid, wherein said sequences flank a variable nucleic acid sequence of said bioagent in said environmental sample;

    producing an amplification product of said variable nucleic acid sequence;

    determining a first molecular mass of said amplification product; and

    comparing said first molecular mass to the molecular masses of known bioagents, thereby identifying the unknown bioagent in said environmental sample.

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