Polymerase compositions

• US 20030228589A1
• Filed: 02/11/2003
• Published: 12/11/2003
• Est. Priority Date: 02/12/2002
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Bond-Nutter, Diane R., Rozzelle, James E., Heiner, Cheryl R.
• Application Number:
  US10/365,032
• Publication Number:
  US 20030228589A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12Y 207/07007 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

Compositions comprising at least two polymerases are provided. Methods for producing primer extension products using at least two polymerases are also provided. Kits for producing primer extension products comprising at least two polymerases are also provided.

Claims

1. A composition comprising Thermus thermophilus GK24 (G46D;

F669Y) and Thermus oshimai (G43D;

F665Y).

2. A method of sequencing a target nucleic acid template comprising:

(a) forming a composition comprising a target nucleic acid template, at least one primer, at least one extendable nucleotide, at least one terminator, Thermus thermophilus GK24 (G46D;

F669Y) and Thermus oshimai (G43D;

F665Y);

(b) incubating the composition under appropriate conditions to generate at least one primer extension product;

(c) separating the at least one primer extension product, wherein the separating comprises at least one mobility-dependent analysis technique (MDAT);

(d) detecting the at least one primer extension product; and

(e) determining the sequence of the target nucleic acid template.

3. A kit comprising Thermus thermophilus GK24 (G46D;

F669Y) and Thermus oshimai (G43D;

F665Y).