Method to enhance homologous recombination

• US 20030228608A1
• Filed: 04/15/2003
• Published: 12/11/2003
• Est. Priority Date: 04/16/2002
• Status: Abandoned Application

• Current Assignee: Promega Corporation
• Original Assignee: Promega Corporation
• Inventors: Grinter, Nigel John, Friedman-Ohana, Rachel, Slater, Michael R.
• Application Number:
  US10/414,729
• Publication Number:
  US 20030228608A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 15/102 Mutagenizing nucleic acids
• CPC Class Codes: C12N 15/1082 Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
• CPC Class Codes: C12N 15/902 using homologous recombination

Abstract

The invention relates to methods for enhancing the targeting of exogenous polynucleotides to a preselected target sequence in a target cell or in an extrachromosomal sequence.

Claims

1. A method for targeting and altering, by homologous recombination, a preselected target nucleic acid sequence in an extrachromosomal sequence, comprising:

a) providing a mixture comprising recombinase and an at least partially single stranded nucleic acid substrate for recombination comprising two nucleic acid molecules, wherein the first and the second nucleic acid molecules each comprise targeting polynucleotides which substantially correspond to or are substantially complementary to the preselected target nucleic acid sequence, wherein the two nucleic acid molecules are capable of forming a partially double stranded molecule with each other, and wherein at least the 5′

end or the 3′

end of the first nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 3′

end or 5′

end of the second nucleic acid molecule, which nucleotide sequence is capable of binding recombinase;

b) contacting the mixture with the extrachromosomal sequence to form a recombination intermediate; and

c) introducing the recombination intermediate into a cell to yield an altered cell comprising a genetically altered extrachromosomal sequence comprising a targeted sequence alteration.

5. The method of claim 1 or 3 wherein the 5′

end of the first nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 3′

end of the second nucleic acid molecule and wherein the 5′

end of the second nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 3′

end of the first nucleic acid molecule, which nucleotide sequences are capable of binding recombinase.

6. The method of claim 1 or 3 wherein the 3′

end of the first nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 5′

end of the second nucleic acid molecule and wherein the 3′

end of the second nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 5′

end of the first nucleic acid molecule, which nucleotide sequences are capable of binding recombinase.

7. The method of claim 1 or 3 wherein the partially single stranded nucleic acid substrate is also partially double stranded.

11. The method of claim 1 or 3 wherein the nucleic acid substrate comprises two single stranded nucleic acid molecules.

12. The method of claim 1, 2, 3, or 4 wherein the mixture is introduced into the cell by lipofection, transfection, microinjection, electroporation, laser poration, biolistics or calcium-mediated transformation.

13. The method of claim 1, 2, 3, or 4 wherein each targeting polynucleotide is greater than 20 nucleotides in length.

14. The method of claim 1, 2, 3, or 4 wherein at least one of the nucleic acid molecules comprises a plurality of deletions, a plurality of additions, a plurality of substitutions, or any combination thereof, relative to the preselected target nucleic acid sequence.

15. The method of claim 1, 2, 3, or 4 wherein the nucleotide sequence comprises targeting polynucleotides.

2. A method for targeting and altering, by homologous recombination, a preselected target nucleic acid sequence in an extrachromosomal sequence, comprising:

a) providing a mixture comprising recombinase and a nucleic acid substrate for recombination comprising two nucleic acid molecules which together form a substantially double stranded molecule having single stranded 5′ and

3′

ends, wherein the first and the second nucleic acid molecules each comprise targeting polynucleotides which substantially correspond to or are substantially complementary to the preselected target nucleic acid sequence, and wherein at least one of the single stranded ends is capable of binding recombinase;

b) contacting the mixture with the extrachromosomal sequence to form a recombination intermediate; and

c) introducing the recombination intermediate into a cell to yield an altered cell comprising a genetically altered extrachromosomal sequence comprising a targeted sequence alteration.

8. The method of claim 2 or 4 wherein the single stranded ends are formed using helicase.

3. A method for targeting and altering, by homologous recombination, a preselected target nucleic acid sequence in a cell, comprising:

a) providing a mixture comprising recombinase and an at least partially single stranded nucleic acid substrate for recombination comprising two nucleic acid molecules, wherein the first and the second nucleic acid molecules each comprise targeting polynucleotides which substantially correspond to or are substantially complementary to the preselected target nucleic acid sequence, wherein the two nucleic acid molecules are capable of forming a partially double stranded molecule with each other, and wherein at least the 5′

end or the 3′

end of the first nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 3′

end or the 5′

end of the second nucleic acid molecule, which nucleotide sequence is capable of binding recombinase; and

b) contacting a cell with the mixture to yield an altered cell comprising a targeted sequence alteration.

9. The method of claim 3 or 4 wherein the preselected target nucleic acid sequence is an extrachromosomal sequence present in the cell to be altered.

10. The method of claim 3 or 4 wherein the preselected target nucleic acid sequence is a chromosomal sequence of the cell.

4. A method for targeting and altering, by homologous recombination, a preselected target nucleic acid sequence in a cell, comprising:

a) providing a mixture comprising recombinase and a nucleic acid substrate for recombination comprising two nucleic acid molecules which together form a substantially double stranded molecule having single stranded 5′ and

3′

ends, wherein the first and the second nucleic acid molecules each comprise targeting polynucleotides which substantially correspond to or are substantially complementary to the preselected target nucleic acid sequence, and wherein at least one of the single stranded ends is capable of binding recombinase; and

b) contacting a cell with the mixture to yield an altered cell comprising a targeted sequence alteration.

16. A method of generating a library of recombination intermediates comprising variant nucleic acid sequences of a preselected target nucleic acid sequence in an extrachromosomal sequence, comprising adding to the extrachromosomal sequence, recombinase and a plurality of at least partially single stranded nucleic acid substrates for recombination, to form a library of recombination intermediates, wherein each substrate comprises two variant nucleic acid molecules, wherein the first and the second variant nucleic acid molecules each comprise targeting polynucleotides which substantially correspond to or are substantially complementary to the preselected target nucleic acid sequence, wherein the two variant nucleic acid molecules are capable of forming a partially double stranded molecule with each other, wherein at least the 5′

end or the 3′

end of the first variant nucleic acid molecule comprises a nucleotide sequence, the substantial complement of which is not present at the 3′

end or 5′

end of the second variant nucleic acid molecule, which nucleotide sequence is capable of binding recombinase, and wherein the plurality of substrates comprise a library of mismatches between the targeting polynucleotides and the target nucleic acid sequence.

17. The method of claim 16 further comprising introducing the library into cells.

18. The method of claim 17 wherein the cells are prokarylotic cells.

19. The method of claim 17 wherein the cells are eukaryotic cells.