Nucleic acid memory device

• US 20030228611A1
• Filed: 05/01/2003
• Published: 12/11/2003
• Est. Priority Date: 05/01/2002
• Status: Abandoned Application

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Chruch, George M., Shendure, Jay
• Application Number:
  US10/427,745
• Publication Number:
  US 20030228611A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2521/507 Recombinase

Abstract

This invention pertains to methods of imparting information onto nucleic acid sequences. In specific embodiments, the present invention provides site-specific recombinase systems and error-prone polymerase systems to alter nucleotide sequences such as DNA and mini-genomes, as well as for the production of microarrays. The present invention also provides methods of analyzing the modified nucleotides sequences provided herein. Methods of engineering and screening for novel modified polymerases that incorporate chain terminating nucleotides and/or labeled nucleotides more efficiently than wild-type polymerases are also provided.

Claims

1. A method of using a nucleic acid sequence for encoding information comprising:

a) providing a nucleic acid sequence including a central portion between two binding domains, wherein the central portion is capable of having its position altered on the nucleic acid between two states or eliminated from the nucleic acid when the binding domains are contacted with a site-specific recombinase specific for the binding domains;

b) contacting the two binding domains with the site-specific recombinase; and

c) allowing the site-specific recombinase to alter the position of or eliminate the central portion between the two binding domains.

2. The method of claim 1, wherein the position of the central portion between the two binding domains is irreversibly altered.

3. The method of claim 1, wherein the site-specific recombinase is Flp recombinase.

4. The method of claim 1, wherein the site-specific recombinase is under the control of an inducible promoter.

5. The method of claim 1, wherein the nucleic acid sequence comprises a mini-genome.

6. A method of using an immobilized nucleic acid array for encoding information comprising:

a) attaching a nucleic acid sequence including a central portion between two binding domains, wherein the central portion is capable of having its position altered on the nucleic acid between two states or eliminated from the nucleic acid when the binding domains are contacted with a site-specific recombinase specific for the binding domains on a substrate;

b) contacting the substrate with the site-specific recombinase; and

c) allowing the site-specific recombinase to alter the position of or eliminate the central portion between the two binding domains.

7. A method of analyzing a degree of evolutionary divergence between organisms comprising:

a) providing a first organism expressing a nucleic acid sequence including multiple regions having a central portion between two binding domains, wherein the central portion is capable of having its position altered on the nucleic acid between two states or removed from the nucleic acid when the binding domains are contacted with a site-specific recombinase specific for the binding domains;

b) providing a second organism expressing the nucleic acid sequence in step a);

c) growing the organisms for a specific length of time;

d) analyzing the nucleic acid sequence from the first organism to determine the pattern of central portions states;

e) analyzing the nucleic acid sequence from the second organism to determine the pattern of central portion states; and

f) comparing the pattern of the central portion states to determine the degree of evolutionary divergence between the organisms.

8. The method of claim 7, wherein three or more organisms are compared.

9. The method of claim 7, wherein the position of the central portion between the two binding domains is irreversibly altered.

10. The method of claim 7, wherein the nucleic acid sequence comprises a mini-genome.

11. A method of recording transcriptional activation of a nucleic acid sequence comprising:

a) providing a nucleic acid sequence including one or more regions having a central portion between two binding domains, wherein the central portion is capable of having its position altered on the nucleic acid between two states or removed from the nucleic acid when the binding domains are contacted with a site-specific recombinase specific for the binding domains;

b) providing a nucleic acid encoding the site-specific recombinase under the control of an inducible promoter; and

c) inducing the promoter;

d) wherein promoter induction results in the central portion between the two binding domains having its position altered or removed.

12. A method of synthesizing an array of nucleic acids on a substrate comprising:

a) providing a substrate;

b) placing a plurality of nucleic acid primers on the substrate;

c) contacting the primers with an error-prone polymerase and a reversible chain terminating nucleotide, wherein the polymerase adds the nucleotide to the primer;

d) exposing a selected region to an effector that modifies the nucleotide such that it is not a chain terminating nucleotide;

e) repeating steps c)-d).

13. The method of claim 12, wherein reversible chain terminating nucleotide is photo-reversible.

14. The method of claim 12, wherein the effector is light.

15. The method of claim 12, wherein the array is analyzed by polony fluorescent in situ sequencing.

16. The method of claim 12, wherein the array is analyzed by rolling circle amplification.

17. A method of synthesizing an array of nucleic acids on a substrate comprising:

a) providing a substrate;

b) placing a plurality of nucleic acid primers on selected regions of the substrate;

c) contacting the primers with an error-prone polymerase and a reversible chain terminating nucleotide, wherein the polymerase adds the nucleotide to the primer;

d) exposing the substrate to an effector that modifies the nucleotide such that it is not a chain terminating nucleotide; and

e) repeating steps c)-d).

18. The method of claim 17, wherein reversible chain terminating nucleotide is photo-reversible.

19. The method of claim 17, wherein the effector is light.

20. The method of claim 17, wherein the array is analyzed by polony fluorescent in situ sequencing.

21. The method of claim 17, wherein the array is analyzed by rolling circle amplification.

22. A method of using a nucleic acid sequence for encoding sensor information comprising:

a) providing a sensor, wherein the sensor is altered when contacted by a ligand such that the altered sensor can be incorporated into a nucleotide sequence by an error-prone polymerase;

b) contacting the sensor with a ligand to produce an altered sensor;

c) contacting the altered sensor with an error-prone polymerase, wherein the polymerase adds the sensor to the nucleic acid sequence.

23. The method of claim 22, wherein the sensor is a photo-activated nucleotide.

24. The method of claim 22, wherein the ligand is light.

25. The method of claim 22, wherein the ligand is a metabolic product.

26. The method of claim 22, wherein the ligand is a chemical.

27. The method of claim 22, wherein the nucleic acid sequence encoding sensor information is analyzed by polony fluorescent in situ sequencing.

28. The method of claim 22, wherein the nucleic acid sequence encoding sensor information is analyzed by rolling circle amplification.

29. A method of using a nucleic acid sequence for encoding sensor information comprising:

a) providing a nucleic acid sequence encoding an allosteric ribozyme sensor comprising a downstream gene, wherein the ribozyme is triggered to cleave itself out of the nucleic acid sequence when contacted by a ligand such that the downstream gene is translated at increased levels; and

b) contacting the nucleic acid sequence with the ligand such that the ribozyme is triggered to cleave itself.

30. The method of claim 29, wherein the downstream gene encodes green fluorescent protein.

31. The method of claim 29, wherein the downstream gene is translated at decreased levels after ribozyme cleavage.

32. A method for identifying a modified polymerase that incorporates a chain terminating nucleotide into a nucleic acid polymer at greater than wild-type levels comprising:

a) providing a wild-type polymerase;

b) providing at least one modified polymerase;

c) contacting each polymerase with nucleic acid templates;

d) contacting each polymerase with reversible chain terminating nucleotides and allowing the polymerases to add a chain terminating nucleotide to the templates for a specific amount of time;

e) measuring the rate of chain terminating nucleotide incorporation for each polymerase, wherein a modified polymerase capable of incorporating a chain terminating nucleotide will incorporate the nucleotides at a faster rate than the wild-type polymerase; and

f) identifying the modified polymerase.

33. A method for identifying a modified polymerase that incorporates a labeled nucleotide triphosphate into a nucleic acid polymer at greater than wild-type levels comprising:

a) providing a wild-type polymerase;

b) providing at least one modified polymerase;

c) contacting each polymerase with nucleic acid templates;

d) contacting each polymerase with labeled nucleotide triphosphates and allowing the polymerases to add labeled nucleotide triphosphates to the templates for a specific amount of time; and

e) measuring the amount of labeled nucleotide triphosphate incorporation for each polymerase, and identifying a modified polymerase capable of incorporating more labeled nucleotide triphosphate than the wild-type polymerase.