Carrier for cell culture

• US 20030228693A1
• Filed: 03/11/2003
• Published: 12/11/2003
• Est. Priority Date: 03/12/2002
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Tsuzuki, Hirohiko, Matsuura, Akiko
• Granted Patent: US 7,041,505 B2
• Time in Patent Office: Days
• US Class Current: 435/397
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 2533/74 Alginate
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0688 Cells from the lungs or the respiratory tract

Abstract

A carrier for cell culture comprising an alginic acid gel layer laminated with a gel layer containing a cell adhesion substance, wherein the gel layer containing a cell adhesion substance has a dry thickness of less than 0.3 μm. The carrier for cell culture enables reproducible and convenient cell layer lamination without inhibiting growth and proliferation of cells upon solubilization of the carrier for preparation of a cell sheet.

Claims

1. A carrier for cell culture comprising an alginic acid gel layer laminated with a gel layer containing a cell adhesion substance, wherein the gel layer containing a cell adhesion substance has a dry thickness of less than 0.3μ

m.

2. The carrier for cell culture according to claim 1, wherein the dry thickness of the alginic acid gel layer is from 0.1 to 10 μ

m.

3. The carrier for cell culture according to claim 1, wherein the gel layer containing a cell adhesion substance is formed by immersing the alginic acid gel layer in a solution containing a cell adhesion substance.

4. The carrier for cell culture according to claim 1, which comprises a porous membrane and an alginic acid gel layer formed on the porous membrane.

5. The carrier for cell culture according to claim 1, wherein the alginic acid gel is a calcium alginate gel.

6. A method for cell culture, which comprises the step of culturing cells by using the carrier for cell culture according to claim 1.

7. A cell culture obtained by the method according to claim 6, wherein said cell culture has a cell layer formed on a surface of the gel layer containing a cell adhesion substance.

8. A cell culture obtained by subjecting the alginic acid gel layer of the cell culture according to claim 7 to a solubilization treatment, wherein said cell culture has a cell layer formed on a surface of the gel layer containing a cell adhesion substance.

9. The cell culture according to claim 8, wherein the solubilization treatment is performed by culturing the cell culture using a medium containing phosphoric acid, and wherein the medium is substantially free from polyvalent metal cations, or the medium contains a chelating agent in an amount of 90 mol % or more based on the total molar amount of polyvalent metal cations.

10. A method for transferring and adhering a cell layer of the cell culture according to claim 7 to a surface of a substrate, which comprises the step of performing a culture under contact with pressure of the cell layer and the substrate.

11. The method according to claim 10, which further comprises the step of solubilization treatment of the alginic acid gel layer.

12. A method for laminating a cell layer of the cell culture according to claim 7 on other cell layer, which comprises the step of performing culture under contact with pressure of the cell layer of said cell culture and the other cell layer.

13. The method according to claim 12, which further comprises the step of solubilization treatment of the alginic acid gel layer.

14. A cell culture comprising laminated cell layers obtainable by the method according to claim 13.