Coated surfaces for immobilizing negatively charged anticoagulating agents from blood fluid

• US 20030229376A1
• Filed: 03/14/2003
• Published: 12/11/2003
• Est. Priority Date: 06/01/1999
• Status: Active Grant

• Current Assignee: BioInteractions Ltd., CloSys, Inc.
• Original Assignee: BioInteractions Ltd.
• Inventors: Sandhu, Shivpal S.
• Granted Patent: US 8,263,223 B2
• Time in Patent Office: Days
• US Class Current: 606/214
• CPC Class Codes: A61B 17/00491 Surgical glue applicators surgical adhesives A61L24/00; two-component delivery syringes A61M5/19
• CPC Class Codes: A61B 17/0057 Implements for plugging an opening in the wall of a hollow or tubular organ, e.g. for sealing a vessel puncture or closing a cardiac septal defect sutures A61B17/04; closing rectum or urethra near body surface A61F2/0009
• CPC Class Codes: Y10T 428/2996 Glass particles or spheres
• CPC Class Codes: Y10T 428/31928 Ester, halide or nitrile of addition polymer

Abstract

A wound closure apparatus is provided which utilizes blood fluid by activating the clotting cascade of blood fluid outside the body within a substantially enclosed sterile container then introducing, the blood fluid to the wound site to complete clotting. An apparatus for providing ways of inhibiting anticoagulating agents, and slowing fibrin clot degradation are also disclosed. Kits for practicing the invention singularly or in combination with, and/or associated with preferred procedures are also disclosed. The invention provides a clotting cascade initiation apparatus (1) including a substantially enclosed sterile containment chamber within which an aliquot of blood fluid, either autologous or from donor sources can be received, and retained. In preferred embodiments, the sterile containment chamber further includes a heparin binding agent which will bind heparin and remove it from the blood fluid. In further embodiments, the containment chamber will also include a procoagulating agent, wherein a clotting cascade can be initiated when the blood fluid is accepted into the sterile containment chamber.

Claims

1. A coating for a surface comprising:

at least a first coating, a second coating, and a third coating, wherein the first coating is in contact with at least a portion of the surface and the second coating is disposed between the first and the third coating,

the first and third coatings comprising polyethyleneimine and the second coating comprising a high molecular weight negatively charged polymer.

2. The coating of claim 1, wherein the polyethyleneimine comprises polyethyleneimine derivatized by butylation.

3. The coating of claim 1, wherein the high molecular weight negatively charged polymer is polyacrylic acid with a molecular weight of at least 3,000,000.

4. The coating of claim 1, wherein the second coating is a coating made from a 0.06% w/v solution of polyacrylic acid in aqueous solution.

5. A method for coating a surface comprising:

coating at least a portion of the surface with a first coating of a positively charged polymer;

coating at least a portion of the first coating with a second coating that contacts the first coating, wherein the second coating includes a high molecular weight negatively charged polymer; and

coating at least a portion of the second coating with a third coating of a positively charged polymer that contacts the second coating.

6. The method of claim 5, wherein the first and third coatings comprise polyethyleneimine.

7. The method of claim 5, wherein the high molecular weight negatively charged polymer comprises polyacrylic acid having a molecular weight of at least 3,000,000.

8. The method of claim 7, wherein the second coating is a coating made from a 0.06% w/v solution of polyacrylic acid in aqueous solution.

9. A coated surface for immobilizing negatively charged anti-coagulating agents from a blood fluid, the coated surface comprising;

a surface, and at least a first layer, a second coating, and a third coating,

wherein the first layer is chosen from the group consisting of a positively charged polymer coating and positively charged functional groups;

the second coating comprises a negatively charged high molecular weight polymer disposed between the first layer and the third coating; and

the third coating comprising polyethyleneimine.

10. The surface of claim 9, wherein the polyethyleneimine is butylated.

11. The surface of claim 9, wherein the negatively charged high molecular weight polymer is polyacrylic acid with a molecular weight of at least 3,000,000.

12. The surface of claim 9, wherein the second coating is a coating made from a 0.06% w/v solution of polyacrylic acid in aqueous solution.

13. The surface of claim 9, wherein the surface is a porous matrix.

14. The surface of claim 13, wherein the surface is a plurality of glass beads.

15. The surface of claim 9, wherein the first layer and the surface are identical and the first layer comprises positively charged functional groups.