Method and apparatus for bonded fluidic circuit for optical bio-disc

• US 20030230383A1
• Filed: 07/24/2002
• Published: 12/18/2003
• Est. Priority Date: 07/24/2001
• Status: Abandoned Application

• Current Assignee: Vindur Technologies, Inc.
• Original Assignee: Vindur Technologies, Inc.
• Inventors: Sasaki, Glenn
• Application Number:
  US10/205,011
• Publication Number:
  US 20030230383A1
• Time in Patent Office: Days
• US Class Current: 156/321
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B29C 65/02 by heating, with or without pressure
• CPC Class Codes: B29C 65/08 using ultrasonic vibrations non-plastics element to plastics elements B29C65/645
• CPC Class Codes: B29C 65/1406 Ultraviolet [UV] radiation
• CPC Class Codes: B29C 65/48 using adhesives , i.e. using supplementary joining material; solvent bonding
• CPC Class Codes: B29C 65/4825 Pressure sensitive adhesives
• CPC Class Codes: B29C 65/4835 Heat curing adhesives
• CPC Class Codes: B29C 65/4845 Radiation curing adhesives, e.g. UV light curing adhesives
• CPC Class Codes: B29C 66/026 Chemical pre-treatments B29C66/028 takes precedence
• CPC Class Codes: B29C 66/54 Joining several hollow-preforms, e.g. half-shells, to form hollow articles, e.g. for making balls, containers; Joining several hollow-preforms, e.g. half-cylinders, to form tubular articles
• CPC Class Codes: B29L 2031/756 Microarticles, nanoarticles
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

Embodiments of the present invention are directed to a method and apparatus for bonded fluidic circuit for an optical bio-disc. In one embodiment of the present invention, a bio-disc is formed using at least two discs. In one embodiment, a shim is used to bond the two discs. In another embodiment, ultra-violet (UV) cured adhesives are used to bond the two discs. In yet another embodiment, the two discs are welded together using ultrasonic energy. In one embodiment, flash chambers containing a fluid are included in the fluidic circuit. During use of the bio-disc, the flash chambers are heated using a laser, causing the fluid to expand and/or vaporize. The expansion of the fluid in the flash chamber is used to propel the sample fluid through the fluidic circuit as desired.

Claims

1. A method of forming a bio-disc comprising:

creating channels in a shim; and

bonding an upper disc to a lower disc using said shim.

2. The method of claim 1 wherein said step of bonding comprises:

heating said shim.

3. The method of claim 1 wherein said step of bonding comprises:

pressing said upper disc against said shim; and

pressing said lower disc against said shim.

4. The method of claim 1 further comprising:

forming a fluidic circuit component in said upper disc.

5. A method of forming a bio-disc comprising:

forming a fluidic circuit in an upper disc; and

bonding said upper disc to a lower disc using an adhesive.

6. The method of claim 5 further comprising:

applying said adhesive to said upper disc.

7. The method of claim 6 further comprising:

modifying a region of said upper disc to make said region hydrophilic,

wherein said adhesive is hydrophilic.

8. The method of claim 6 further comprising:

modifying a region of said upper disc to make said region hydrophobic,

wherein said adhesive is hydrophobic.

9. The method of claim 6 further comprising:

charging a region of said upper disc; and

covalently bonding said adhesive to said region.

10. The method of claim 6 further comprising:

placing a mask on said upper disc before said adhesive is applied; and

removing said mask after said adhesive is applied.

11. The method of claim 5 further comprising:

curing said adhesive using ultra-violet light.

12. A method of controlling fluid flow in a bio-disc comprising:

heating a first fluid in a fluidic circuit with a laser; and

displacing a second fluid in said fluidic circuit wherein said second fluid is displaced by the expansion of said first fluid caused by said step of heating.

13. The method of claim 12 wherein said step of displacing causes said second fluid to move through said fluidic circuit closer to a center of said bio-disc;

14. The method of claim 13 further comprising:

spinning said bio-disc to cause said second fluid to move away from said center through said fluidic circuit using centrifugal force.

15. The method of claim 12 further comprising:

washing said fluidic circuit with a third fluid wherein said step of washing removes an unwanted substance from an analysis region of said fluidic circuit.

16. A bio-disc comprising:

a shim wherein a channel is formed in said shim;

an upper disc; and

a lower disc wherein said upper disc is bonded to said lower disc using said shim.

17. The bio-disc of claim 16 wherein heat is applied to said shim to bond said upper disc to said lower disc.

18. The bio-disc of claim 16 wherein said upper disc and said lower disc are pressed against said shim to bond said upper disc to said lower disc.

19. The bio-disc of claim 16 further comprising:

a fluidic circuit component formed in said upper disc.

20. A bio-disc comprising:

an upper disc;

a fluidic circuit in said upper disc;

a lower disc; and

an adhesive configured to bond said upper disc to a lower disc.

21. The bio-disc of claim 20 wherein said adhesive is applied to said upper disc.

22. The bio-disc of claim 21 wherein a region of said upper disc is modified to make said region hydrophilic and wherein said adhesive is hydrophilic.

23. The bio-disc of claim 21 wherein a region of said upper disc is modified to make said region hydrophobic and wherein said adhesive is hydrophobic.

24. The bio-disc of claim 21 wherein a region of said upper disc is charged and wherein said adhesive is covalently bonded to said region.

25. The bio-disc of claim 21 further comprising:

a mask wherein said mask is placed on said upper disc before said adhesive is applied and wherein said mask is removed after said adhesive is applied.

26. The bio-disc of claim 20 wherein said adhesive is cured using ultraviolet light.

27. A fluid flow control system for a bio-disc comprising:

a fluidic circuit;

a laser configured to heat a first fluid in said fluidic circuit causing it to expand wherein a second fluid in said fluidic circuit is displaced by the expansion of said first fluid.

28. The fluid flow control system for a bio-disc of claim 27 wherein said second fluid moves through said fluidic circuit closer to a center of said bio-disc;

29. The fluid flow control system for a bio-disc of claim 28 further comprising:

a rotation system configured to spin said bio-disc to cause said second fluid to move away from said center through said fluidic circuit using centrifugal force.

30. The fluid flow control system for a bio-disc of claim 27 further comprising:

a washing system configured to wash said fluidic circuit with a third fluid wherein said third fluid removes an unwanted substance from an analysis region of said fluidic circuit.