Microfluidic device preparation system

• US 20030230488A1
• Filed: 06/13/2002
• Published: 12/18/2003
• Est. Priority Date: 06/13/2002
• Status: Abandoned Application

• Current Assignee: Aclara Biosciences, Inc (Laboratory Corporation Of America Holdings)
• Original Assignee: Aclara Biosciences, Inc (Laboratory Corporation Of America Holdings)
• Inventors: Bjornson, Torlief O., Lee, Lawrence
• Application Number:
  US10/172,171
• Publication Number:
  US 20030230488A1
• Time in Patent Office: Days
• US Class Current: 204/453
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates

Abstract

Devices, systems and methods for filling and processing microfluidic devices are disclosed. A device of the system comprising a priming block is disclosed, that functions to drive fluid into a plurality of separation networks in a microfluidic device, using air pressure or electromotive force. The priming block may be incorporated into a microfluidic device preparation system additionally comprising a platform for positionally holding the microfluidic device and a sample array and fluid dispensing modules that are movable in any direction relative to the platform of the microfluidic device. The preparation system may be further incorporated into a greater system that performs processing functions either preceding or following the preparation system, including sample array preparation, and processing and analysis of the samples contained in the prepared microfluidic devices. Methods for operation of the various components of the disclosed systems are also provided.

Claims

1. A priming block for filling a plurality of fluid networks contained in a microfluidic device, each fluid network being externally and fluidly accessible through a priming reservoir, the priming block comprising:

(a) means for operatively connecting with a plurality of the priming reservoirs, and

(b) means for driving fluid into the plurality of fluid networks upon making the operative connection, thereby to fill the plurality of fluid networks.

2. The priming block of claim 1, wherein said means for driving fluid comprises at least one of air pressure and electromotive force.

3. The priming block of claim 2, wherein said air pressure further comprises:

(a) a source of pressurized air;

(b) a pressure line connecting the source of pressurized air to said priming block; and

(c) a valve within the pressure line that regulates delivery of pressurized air from the source to said priming block.

4. The priming block of claim 2, wherein said electromotive force further comprises an electrically grounded plane positioned against the face of said microfluidic device opposite of said priming reservoir.

5. A system for filling a plurality of fluid networks contained in a microfluidic device, each fluid network being externally and fluidly accessible through a priming reservoir, the system comprising:

(a) a platform for positionally holding the microfluidic device; and

(b) a priming block comprising;

(i) means for operatively connecting with a plurality of the priming reservoirs when the microfluidic device is positioned on the platform, and

(ii) means for driving fluid into the plurality of fluid networks upon making the operative connection, thereby to fill the plurality of fluid networks.

6. The system of claim 5, wherein said platform further comprises means for regulating the temperature at said position for said microfluidic device.

7. The system of claim 5, wherein said means for driving fluid comprises at least one of air pressure and electromotive force.

8. The system of claim 7, wherein:

said means for driving fluid comprising air pressure further comprises

(a) a source of pressurized air,

(b) a pressure line connecting the source of pressurized air to said priming block, and

(c) a valve within the pressure line that regulates delivery of pressurized air from the source to said priming block;

and said means for operatively connecting comprises a compressible material on the connecting surface of said priming block, the compressible material providing a pressurized air seal between said priming block and said external openings of said priming reservoirs.

9. The system of claim 7, wherein:

said means for operatively connecting comprises a plurality of electrodes, each inserted by said priming block into one of said plurality of priming reservoirs, and

said means for driving fluid comprising electromotive force further comprises

(a) an electrically grounded plane positioned against the face of said microfluidic device on the opposite side of said priming reservoir, and

(b) a source of power that can be delivered to the electrodes.

10. The system of claim 5, further comprising:

(a) a gantry positioned above said platform, wherein said platform and the gantry are moveable relative to each other along a first axis;

(b) a carriage moveably mounted to the gantry, where the carriage motion is along an axis perpendicular to the first axis;

(c) a first moveable mounting means for mounting the carriage to the gantry, where the carriage motion is along an axis perpendicular to the first axis; and

(d) a second moveable mounting means for mounting said priming block to the carriage, where the motion on the carriage is in an axis perpendicular to the plane of said platform.

11. The system of claim 5, further comprising means for determining the operativity of each of the filled fluid networks for electrophoretic separations.

12. The system of claim 11, wherein the means for determining comprises components for monitoring a property of said microchannels, the property being one from the group including resistive, capacitive, optical, transmissive, sonic and ultrasonic properties.

13. The system of claim 12 for monitoring said property of resistance, the components comprising a power source for applying a voltage across said filled fluid networks, and a detector for monitoring the current generated by the voltage.

14. The system of claim 12 for monitoring said property of transmission, the components comprising a light source for directing light across said filled fluid networks, and a detector for monitoring the light transmitted across said filled fluid networks, wherein the light source and the detector are disposed on opposite sides of said fluid networks.

15. The system of claim 5, further comprising means for automated operation of said system.

16. The system of claim 5, wherein said platform is further functional to positionally hold both said microfluidic device and a sample array, and the system further comprises at least one fluid dispensing module that is movable to operative positions relative to said platform.

17. The system of claim 16, wherein said platform further comprises means for separately regulating the temperature at said positions for said microfluidic device and said sample array.

18. The system of claim 16, further comprising means for regulating the temperature of said fluid-dispensing module.

19. The system of claim 16, further comprising a wash station functionally accessible to said fluid dispensing module.

20. The system of claim 19, further comprising means for heating said wash station.

21. The system of claim 19, further comprising means for providing a flow of rinse liquid.

22. The system of claim 16, further comprising:

(a) a gantry positioned above said platform, wherein said platform and the gantry are moveable relative to each other along a first axis;

(b) a carriage;

(c) a first moveable mounting means for mounting the carriage to the gantry, where the carriage motion is along an axis perpendicular to the first axis; and

(d) a second moveable mounting means for mounting at least one of said fluid dispensing module and said priming block to the carriage, where the motion on the carriage is in an axis perpendicular to the plane of said platform.

23. The system of claim 22, further comprising a fluid-containing cartridge providing fluid to said fluid-dispensing module.

24. The system of claim 22, wherein said carriage has a hinged compartment fitted to receive said fluid-containing cartridge.

25. The system of claim 16, wherein said fluid dispensing module is selected from the group consisting of:

(a) an aspiration/dispense unit comprising a plurality of tips, the end of each tip movable into functional proximity to at least one of (i) a well in a sample array positioned on said platform and (ii) a reservoir on a microfluidic device positioned on said platform; and

(b) a separation medium dispenser having at least one medium dispensing line, each dispensing line terminating at a medium dispense tip that is movable into functional proximity to said priming reservoirs on said microfluidic device positioned on said platform.

26. The system of claim 25, wherein said aspiration/dispense unit further comprises eight tips mounted in parallel with a spacing of 9 mm between adjacent tips.

27. The system of claim 25, wherein said aspiration/dispense tips operate by positive displacement.

28. The system of claim 16, further comprising at least one from the group consisting of:

(a) a sample preparation module functional to prepare said sample array,

(b) a separation/detection module functional to separate components contained within said microfluidic device and detect the separated components, and

(c) an analysis module functional to collect and analyze data obtained from the detecting.

29. The system of claim 28, further comprising automated means for transferring between said modules either of said sample array and said microfluidic device.

30. A method for preparing a plurality of separation networks in a microfluidic device for a separation, each separation network being externally and fluidly accessible through a priming reservoir and a sample reservoir, comprising the steps of:

(a) dispensing separation medium into one or more of the priming reservoirs fluidly connected to a plurality of the separation networks;

(b) sealing a priming block against the one or more priming reservoirs;

(c) driving fluid into the plurality of separation networks with the priming block to fill the separation networks; and

(d) transferring a plurality of samples from a sample array to the sample reservoirs in fluid connection with the plurality of filled separation networks, thereby preparing the plurality of separation networks contained in the microfluidic device for a separation.

31. The method of claim 30, wherein said driving is achieved using at least one of air pressure and electromotive force.

32. The method of claim 30, wherein eight separation networks are filled simultaneously.

33. The method of claim 30, further comprising after step (c), the step (c-2) of determining the operativity of each of said filled separation networks for electrophoretic separations.

34. The method of claim 33, wherein said determining step comprises 10 monitoring a property of said separation networks, the property being one from the group including resistive, capacitive, optical, transmission, sonic and ultrasonic properties.

35. The method of claim 33, further comprising repeating steps (a) through (c) if one of said separation networks is determined to be inoperative.

36. The method of claim 33, further comprising the step of recording the position of any separation network determined to be inoperative.

37. The method of claim 33, further comprising the step of discarding said microfluidic device containing one of said separation networks determined to be inoperative.

38. The method of claim 30, further comprising the step of transferring said microfluidic device to an analyzer for separation and analysis of said prepared separation networks.

39. The method of claim 30, conducted automatically.