Microscope, particularly a laser scanning microscope with adaptive optical arrangement
First Claim
1. A microscope, particularly a laser scanning microscope, with an adaptive optical device in the microscope beam path, comprising:
- two reflective adaptive elements, at least one of which is constructed as an adaptive optical element, both of which being oriented with their reflector surface vertical to the optical axes of the microscope beam path;
a polarizing beam splitter having a splitter layer which is located in the vertex of two orthogonal arms of the microscope beam path or two orthogonal portions of a folded microscope beam path, wherein a first adaptive element is associated with one arm and the other adaptive element is associated with a second arm, and a quarter-wave plate being located in each arm between the beam splitter and reflective adaptive element, and a detection device to which the detection light is directed and which is linked to the adaptive elements by evaluating and adjusting devices.
3 Assignments
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Accused Products
Abstract
A microscope, particularly a laser scanning microscope, with an adaptive optical device in the microscope beam path, comprising two reflective adaptive elements, at least one of which is constructed as an adaptive optical element, both of which are oriented with their reflector surface vertical to the optical axes of the microscope beam path, and a polarizing beam splitter whose splitter layer is located in the vertex of two orthogonal arms of the microscope beam path or two orthogonal portions of a folded microscope beam path, wherein a first adaptive element is associated with one arm and the other adaptive element is associated with the second arm, and a quarter-wave plate is located in each arm between the beam splitter and reflective adaptive element, and a detection device to which the detection light is directed and which is linked to the adaptive elements by evaluating and adjusting devices.
56 Citations
17 Claims
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1. A microscope, particularly a laser scanning microscope, with an adaptive optical device in the microscope beam path, comprising:
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two reflective adaptive elements, at least one of which is constructed as an adaptive optical element, both of which being oriented with their reflector surface vertical to the optical axes of the microscope beam path;
a polarizing beam splitter having a splitter layer which is located in the vertex of two orthogonal arms of the microscope beam path or two orthogonal portions of a folded microscope beam path, wherein a first adaptive element is associated with one arm and the other adaptive element is associated with a second arm, and a quarter-wave plate being located in each arm between the beam splitter and reflective adaptive element, and a detection device to which the detection light is directed and which is linked to the adaptive elements by evaluating and adjusting devices. - View Dependent Claims (2, 3)
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4. A microscope, particularly a laser scanning microscope, with an adaptive optical device in the microscope beam path, comprising:
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an adaptive reflective element and a nonadaptive reflective element, both of which being oriented with their reflector surface vertical to the optical axis of the microscope beam path;
a polarizing beam splitter having a splitter layer located in the vertex of two orthogonal arms of the microscope beam path or two orthogonal portions of a microscope beam path which is folded multiple times, wherein the adaptive element is associated with one arm and the nonadaptive element is associated with a second arm, and a quarter-wave plate being located in each arm between the beam splitter and the respective reflective element, and a detection device to which the detection light is directed and which is linked to the adaptive element via an evaluating and adjusting device. - View Dependent Claims (5, 6, 7, 8, 9)
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10. A microscope, particularly a laser scanning microscope, with an adaptive optical device, comprising:
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an adaptive concave mirror which is arranged in a pupil plane of the illumination beam path, an optical beam splitter being associated with the adaptive concave mirror in an intermediate image plane and having a transmittive area and a reflective area in a splitter surface, wherein the illumination light is directed initially to the splitter surface and from the reflective area of the latter to the concave mirror, is focused by the concave mirror onto the transmittive area and passes through the latter to the sample; and
/oran adaptive concave mirror which is arranged in a pupil plane of the detection beam path, an optical beam splitter being associated with the adaptive concave mirror in an intermediate image plane and having a transmittive area and a reflective area in a splitter surface, wherein the detection light is directed initially to the splitter surface and from its reflective area to the concave mirror, is focused by the concave mirror onto the transmittive area and passes through the latter to a detection device; and
evaluating and adjusting devices which are connected to the adaptive element in the illumination beam path and/or to the adaptive element in the detection beam path, wherein the output signals of the detection device are used for adaptive regulation of the wavefront of the illumination light or detection light. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification