Hybridization process for arrays

• US 20030232344A1
• Filed: 06/14/2002
• Published: 12/18/2003
• Est. Priority Date: 06/14/2002
• Status: Abandoned Application

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Schleifer, Arthur, Ostrowski, Magdalena
• Application Number:
  US10/172,892
• Publication Number:
  US 20030232344A1
• Time in Patent Office: Days
• US Class Current: 435/6.18
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence

Abstract

A method of performing a hybridization assay using an assay chamber that includes a form-in-place gasket is described. In one embodiment the method includes mating a cover against a complementary surface on a substrate, wherein the substrate has an array surface on which biomolecules are deposited in an array format. A form-in-place gasket is present on the cover, and when the cover is mated against the complementary surface an assay chamber is formed. The method may further include contacting the array surface with the solution to be tested, disassembling the chamber by removing the cover, processing the array surface, and interrogating the array surface using, e.g. an array reader.

Claims

1. A method of performing an array hybridization experiment comprising:

Contacting a target solution with an array disposed in an assay chamber, wherein the assay chamber is substantially defined by a substrate, a cover, and a form-in-place gasket, the form-in-place gasket positioned between the substrate and the cover, wherein the contacting is done under conditions and for a period of time sufficient to allow specific binding interactions between the target solution and the array, and

Interrogating the array.

2. The method of claim 1, wherein the substrate and the cover are substantially planar.

3. The method of claim 1, wherein the substrate defines a depression in the substrate, the depression defining a well.

4. The method of claim 1, wherein the cover defines a depression in the cover, the depression defining a well.

5. The method of claim 1, further comprising an array substrate disposed within the assay chamber, the array substrate having a surface, wherein the array is disposed on the surface of the array substrate.

6. The method of claim 1, wherein at least one of the substrate or the cover has an interior surface, wherein the molecular array is disposed on the interior surface.

7. The method of claim 1, wherein the chamber further comprises an inlet port.

8. The method of claim 1, wherein the chamber further comprises an outlet port.

9. The method of claim 1, wherein the chamber has no port.

10. The method of claim 1, wherein contacting comprises depositing the target solution into a well defined at least in part by one of the substrate or the cover and placing the other one of the substrate or cover over the well with the form-in-place gasket positioned between the substrate and the cover such that a fluid tight seal is formed by the form-in-place gasket positioned between the substrate and the cover.

11. The method of claim 1, wherein contacting comprises introducing the target solution into the chamber via an inlet port in fluid communication with the chamber.

12. The method of claim 1, wherein the assay chamber is adapted to opening at the form-in-place gasket to allow access to the array.

13. The method of claim 1, wherein the assay chamber is adapted for interrogation of the array without displacing the cover from the substrate.

14. The method of claim 1, wherein the form-in-place gasket has a thickness of between about 10 micrometers and about 1.5 millimeters.

15. The method of claim 1, wherein the form-in-place gasket has a width of between about 100 micrometers and about 3 millimeters.

16. The method of claim 1, further comprising, before interrogating the array,

submerging the array chamber in a wash buffer and, while the array chamber is submerged, displacing the cover from the substrate to allow wash buffer to contact the array.