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Complexity management of genomic DNA by locus specific amplification

  • US 20030232348A1
  • Filed: 10/14/2002
  • Published: 12/18/2003
  • Est. Priority Date: 06/17/2002
  • Status: Active Grant
First Claim
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1. A method of amplifying a collection of target sequences from a nucleic acid sample comprising:

  • generating a collection of capture probes comprising a plurality of different species of primers wherein each species comprises a first common sequence and a 3′

    variable region that is specific for a target sequence in said collection of target sequences, wherein said collection of capture probes is attached to a solid support and the 3′

    end of the capture probes is available for extension;

    fragmenting the nucleic acid sample;

    ligating an adapter to the fragments, said adapter comprising a second common sequence;

    hybridizing the adapter-ligated fragments to the collection of capture probes;

    extending the capture probes; and

    amplifying the extended capture probes with first and second common sequence primers.

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