Glucose sensor and uses thereof
First Claim
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1. A recombinant human glucokinase having decreased catalytic activity but a substantially identical ability to bind glucose relative to a corresponding wild-type human glucokinase.
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Abstract
The present invention provides a glucokinase protein in which the catalytic activity has been disabled in order to enable its use as a glucose sensor. The catalytically disabled glucokinase protein can be used as the glucose sensor in hand-held glucose monitors and in implantable glucose monitoring devices. The glucose sensor can also be incorporated into biomedical devices for the continuous monitoring of glucose and administration of insulin.
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Citations
27 Claims
- 1. A recombinant human glucokinase having decreased catalytic activity but a substantially identical ability to bind glucose relative to a corresponding wild-type human glucokinase.
- 10. An isolated nucleic acid molecule encoding a mutant human glucokinase having decreased catalytic activity but a substantially identical ability to bind glucose relative to a corresponding wild-type human glucokinase.
- 22. A glucose sensor comprising a recombinant human glucokinase having decreased catalytic activity but a substantially identical ability to bind glucose relative to the corresponding wild-type human glucokinase.
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25. A method of determining the level of glucose in a sample comprising:
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(a) contacting said sample with a recombinant human glucokinase having decreased catalytic activity but a substantially identical ability to bind glucose relative to a corresponding wild-type human glucokinase;
(b) measuring a change in a physical characteristic of said recombinant glucokinase; and
(c) correlating said change to the level of glucose in said sample. - View Dependent Claims (26, 27)
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Specification