Expression of microbial proteins in plants for production of plants with improved properties

• US 20030233675A1
• Filed: 02/20/2003
• Published: 12/18/2003
• Est. Priority Date: 02/21/2002
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Goldman, Barry S., Chen, Xianfeng, Cao, Yongwei, Hinkle, Gregory J., Slater, Steven C.
• Granted Patent: US 7,314,974 B2
• Time in Patent Office: Days
• US Class Current: 800/279
• CPC Class Codes: C07K 14/195 from bacteria
• CPC Class Codes: C12N 15/8241 Phenotypically and genetically modified plants via recombinant DNA technology
• CPC Class Codes: C12N 15/8243 involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 15/8251 Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: C12N 15/8273 for drought, cold, salt resistance
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8279 for biotic stress resistance, pathogen resistance, disease resistance
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Recombinant constructs and methods useful for improvement of plants are provided. In particular, recombinant constructs comprising promoters functional in plant cells positioned for expression of polynucleotides encoding polypeptides from microbial sources are provided. The disclosed constructs and methods find use in production of transgenic plants to provide plants, particularly crop plants, having improved properties.

Claims

1. A recombinant DNA construct comprising a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide encoding a polypeptide from a microbial source, and wherein said polynucleotide is selected from the group consisting of:

(a) a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO;

1 through SEQ ID NO;

23687;

(b) a polynucleotide encoding a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;

23688 through SEQ ID NO;

47374;

(c) a polynucleotide having at least 70% sequence identity to a polynucleotide of (a) or (b);

(d) a polynucleotide encoding a polypeptide having at least 80% sequence identity to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;

23688 through SEQ ID NO;

47374, wherein said encoded polypeptide is a functional homolog of said polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;

23688 through SEQ ID NO;

47374.

2. A transformed plant comprising a recombinant DNA construct, wherein said construct comprises a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide encoding a polypeptide from a microbial source, and wherein said polynucleotide is selected from the group consisting of:

(a) a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO;

1 through SEQ ID NO;

23687;

(b) a polynucleotide encoding a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;

23688 through SEQ ID NO;

47374;

(c) a polynucleotide having at least 70% sequence identity to a polynucleotide of (a) or (b);

(d) a polynucleotide encoding a polypeptide having at least 80% sequence identity to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;

23688 through SEQ ID NO;

47374, wherein said encoded polypeptide is a functional homolog of said polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;

23688 through SEQ ID NO;

47374.

3. The transformed plant according to claim 2, wherein said plant is a crop plant.

4. The transformed plant according to claim 3 wherein said plant is maize.

5. The transformed plant according to claim 3 wherein said plant is soybean.

6. A method of producing a transformed plant having an improved property, wherein said method comprises transforming a plant with a recombinant construct comprising a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide associated with said property, and growing said transformed plant, wherein said polynucleotide encodes a polypeptide from a microbial source and is selected from the group consisting of:

(a) a polynucleotide encoding a polypeptide useful for improving plant cold tolerance, wherein said polynucleotide comprises a sequence identified in Table 3B;

(b) a polynucleotide encoding a polypeptide useful for providing increased resistance to plant disease, wherein said polynucleotide comprises a sequence identified in Table 4B;

(c) a polynucleotide encoding a polypeptide useful for improving plant drought tolerance, wherein said polynucleotide comprises a sequence identified in Table 5B;

(d) a polynucleotide encoding a polypeptide useful for manipulating growth rate in plant cells by modification of the cell cycle pathway, wherein said polynucleotide comprises a sequence identified in Table 6B;

(e) a polynucleotide encoding a polypeptide useful for production of plant growth regulators, wherein said polynucleotide comprises a sequence identified in Table 7B;

(f) a polynucleotide encoding a polypeptide useful for improving plant heat tolerance, wherein said polynucleotide comprises a sequence identified in Table 8B;

(g) a polynucleotide encoding a polypeptide useful for improving plant tolerance to herbicides, wherein said polynucleotide comprises a sequence identified in Table 9B;

(h) a polynucleotide encoding a polypeptide useful for increasing the rate of homologous recombination in plants, wherein said polynucleotide comprises a sequence identified in Table 10B;

(i) a polynucleotide encoding a polypeptide useful for improving plant tolerance to extreme osmotic conditions, wherein said polynucleotide comprises a sequence identified in Table 11B;

(j) a polynucleotide encoding a polypeptide useful for improving plant tolerance to pathogens or pests, wherein said polynucleotide comprises a sequence identified in Table 12B;

(k) a polynucleotide encoding a polypeptide useful for modifying seed oil yield and/or content, wherein said polynucleotide comprises a sequence identified in Table 13B;

(l) a polynucleotide encoding a polypeptide useful for modifying seed protein yield and/or content, wherein said polynucleotide comprises a sequence identified in Table 14B;

(m) a polynucleotide encoding a polypeptide useful for yield improvement by modification of carbohydrate use and/or uptake, wherein said polynucleotide comprises a sequence identified in Table 15B;

(n) a polynucleotide encoding a polypeptide useful for yield improvement by modification of nitrogen use and/or uptake, wherein said polynucleotide comprises a sequence identified in Table 16B;

(o) a polynucleotide encoding a polypeptide useful for yield improvement by modification of phosphorus use and/or uptake, wherein said polynucleotide comprises a sequence identified in Table 17B;

(p) a polynucleotide encoding a polypeptide useful for yield improvement by modification of photosynthesis, wherein said polynucleotide comprises a sequence identified in Table 18B;

(q) a polynucleotide encoding a polypeptide useful for yield improvement by providing improved plant growth and development under at least one stress condition, wherein said polynucleotide comprises a sequence identified in Table 19B;

(r) a polynucleotide encoding a polypeptide useful for lignin production, wherein said polynucleotide comprises a sequence identified in Table 20B; and

(s) a polynucleotide encoding a polypeptide useful for galactomannan production, wherein said polynucleotide comprises a sequence identified in Table 21B.

7. The method according to claim 6, wherein said transformed plant is a crop plant.

8. The transformed plant according to claim 7 wherein said plant is maize.

9. The transformed plant according to claim 7 wherein said plant is soybean.

10. A method of producing a transformed plant having an improved property, wherein said method comprises transforming a plant with a recombinant construct comprising a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide comprising coding sequence for a polypeptide associated with said property, and growing said transformed plant, wherein said polynucleotide encodes a polypeptide from a microbial source and wherein said polypeptide is selected from the group consisting of:

(a) a polypeptide useful for improving plant cold tolerance, wherein said polypeptide comprises a sequence identified in Table 3A;

(b) a polypeptide useful for providing increased resistance to plant disease, wherein said polypeptide comprises a sequence identified in Table 4A;

(c) a polypeptide useful for improving plant drought tolerance, wherein said polypeptide comprises a sequence identified in Table 5A;

(d) a polypeptide useful for manipulating growth rate in plant cells by modification of the cell cycle pathway, wherein said polypeptide comprises a sequence identified in Table 6A;

(e) a polypeptide useful for production of plant growth regulators, wherein said polypeptide comprises a sequence identified in Table 7A;

(f) a polypeptide useful for improving plant heat tolerance, wherein said polypeptide comprises a sequence identified in Table 8A;

(g) a polypeptide useful for improving plant tolerance to herbicides, wherein said polypeptide comprises a sequence identified in Table 9A;

(h) a polypeptide useful for increasing the rate of homologous recombination in plants, wherein said polypeptide comprises a sequence identified in Table 10A;

(i) a polypeptide useful for improving plant tolerance to extreme osmotic conditions, wherein said polypeptide comprises a sequence identified in Table 11A;

(j) a polypeptide useful for improving plant tolerance to pathogens or pests, wherein said polypeptide comprises a sequence identified in Table 12A;

(k) a polypeptide useful for modifying seed oil yield and/or content, wherein said polypeptide comprises a sequence identified in Table 13A;

(l) a polypeptide useful for modifying seed protein yield and/or content, wherein said polypeptide comprises a sequence identified in Table 14A;

(m) a polypeptide useful for yield improvement by modification of carbohydrate use and/or uptake, wherein said polypeptide comprises a sequence identified in Table 15A;

(n) a polypeptide useful for yield improvement by modification of nitrogen use and/or uptake, wherein said polypeptide comprises a sequence identified in Table 16A;

(o) a polypeptide useful for yield improvement by modification of phosphorus use and/or uptake, wherein said polypeptide comprises a sequence identified in Table 17A;

(p) a polypeptide useful for yield improvement by modification of photosynthesis, wherein said polynucleotide comprises a sequence identified in Table 18A;

(q) a polypeptide useful for yield improvement by providing improved plant growth and development under at least one stress condition, wherein said polypeptide comprises a sequence identified in Table 19A;

(r) a polypeptide useful for lignin production, wherein said polypeptide comprises a sequence identified in Table 20A; and

(s) a polypeptide useful for galactomannan production, wherein said polynucleotide comprises a sequence identified in Table 21A.

11. The method according to claim 10, wherein said transformed plant is a crop plant.

12. The transformed plant according to claim 11 wherein said plant is maize.

13. The transformed plant according to claim 11 wherein said plant is soybean.

14. A method of producing a plant having an improved property, wherein said method comprises transforming a plant with a recombinant construct comprising a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide comprising coding sequence for a polypeptide associated with said property, and growing said transformed plant, wherein said polynucleotide encodes a polypeptide from a microbial source, and said polypeptide is a functional homolog of a reference polypeptide selected from the group of reference polypeptides consisting of:

(a) a polypeptide useful for improving plant cold tolerance, wherein said polypeptide comprises a sequence identified in Table 3A;

(b) a polypeptide useful for providing increased resistance to plant disease, wherein said polypeptide comprises a sequence identified in Table 4A;

(c) a polypeptide useful for improving plant drought tolerance, wherein said polypeptide comprises a sequence identified in Table 5A;

(d) a polypeptide useful for manipulating growth rate in plant cells by modification of the cell cycle pathway, wherein said polypeptide comprises a sequence identified in Table 6A;

(e) a polypeptide useful for production of plant growth regulators, wherein said polypeptide comprises a sequence identified in Table 7A;

(f) a polypeptide useful for improving plant heat tolerance, wherein said polypeptide comprises a sequence identified in Table 8A;

(g) a polypeptide useful for improving plant tolerance to herbicides, wherein said polypeptide comprises a sequence identified in Table 9A;

(h) a polypeptide useful for increasing the rate of homologous recombination in plants, wherein said polypeptide comprises a sequence identified in Table 10A;

(i) a polypeptide useful for improving plant tolerance to extreme osmotic conditions, wherein said polypeptide comprises a sequence identified in Table 11A;

(j) a polypeptide useful for improving plant tolerance to pathogens or pests, wherein said polypeptide comprises a sequence identified in Table 12A;

(k) a polypeptide useful for modifying seed oil yield and/or content, wherein said polypeptide comprises a sequence identified in Table 13A;

(l) a polypeptide useful for modifying seed protein yield and/or content, wherein said polypeptide comprises a sequence identified in Table 14A;

(m) a polypeptide useful for yield improvement by modification of carbohydrate use and/or uptake, wherein said polypeptide comprises a sequence identified in Table 15A;

(n) a polypeptide useful for yield improvement by modification of nitrogen use and/or uptake, wherein said polypeptide comprises a sequence identified in Table 16A;

(o) a polypeptide useful for yield improvement by modification of phosphorus use and/or uptake, wherein said polypeptide comprises a sequence identified in Table 17A;

(p) a polypeptide useful for yield improvement by modification of photosynthesis, wherein said polynucleotide comprises a sequence identified in Table 18A;

(q) a polypeptide useful for yield improvement by providing improved plant growth and development under at least one stress condition, wherein said polypeptide comprises a sequence identified in Table 19A;

(r) a polypeptide useful for lignin production, wherein said polypeptide comprises a sequence identified in Table 20A; and

(t) a polypeptide useful for galactomannan production, wherein said polynucleotide comprises a sequence identified in Table 21A; and

wherein said polypeptide comprises at least 50 consecutive amino acids having at least 80% identity to a 50 amino acid polypeptide fragment of said reference polypeptide.

15. The method according to claim 14, wherein said transformed plant is a crop plant.

16. The transformed plant according to claim 15 wherein said plant is maize.

17. The transformed plant according to claim 15 wherein said plant is soybean.