Base member for a biochip

• US 20030235905A1
• Filed: 06/12/2003
• Published: 12/25/2003
• Est. Priority Date: 06/22/2002
• Status: Abandoned Application

• Current Assignee: LaVision BioTec GmbH (Miltenyi GmbH)
• Original Assignee: LaVision BioTec GmbH (Miltenyi GmbH)
• Inventors: Spiecker, Heinrich
• Application Number:
  US10/459,854
• Publication Number:
  US 20030235905A1
• Time in Patent Office: Days
• US Class Current: 435/287.1
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50853 with covers or lids

Abstract

A base member for a biochip, e.g., a chip for analyzing DNA, RNA, proteins or cells, the base member (1), which is shaped like a board, being provided with a plurality of discrete elevations (2) extending from the upper side (1a) to the underside and having, in the region of their lower end, an active surface (3) for receiving the probe material.

Claims

1. A base member for a biochip, e.g., a chip for analyzing DNA, RNA, proteins or cells,

characterized in that the base member (1), which is shaped like a board, is provided with a plurality of discrete elevations (2) extending from the upper side (1a) to the underside and having, in the region of their lower end, an active surface (3) for receiving the probe material.

2. The base member according to claim 1,

characterized in that the elevations (2) are configured in the shape of a truncated cone or pyramid.

3. The base member according to claim 1,

characterized in that the active surface (3) is between 50 and 200 ·

m in diameter.

4. The base member according to claim 1,

characterized in that the elevations (2) are disposed in a pattern matching a nanotiter plate (5).

5. The base member according to claim 1,

characterized in that a lens (4) is disposed locally above each elevation (5).

6. An arrangement for reading the chip according to one or several of the claims 1 through 5,

characterized by a detection optics (21).

7. The arrangement according to claim 6,

characterized in that the arrangement comprises a radiation source (13).

8. The arrangement according to claim 7,

characterized in that the radiation source (13) is a white light source, a laser or an array of LEDs or laser diodes.

9. The arrangement according to claim 7,

characterized in that an aperture plate arrangement (17) for screening fractions of scattered light is provided between the radiation source (13) and the chip (1).

10. The arrangement according to claim 9,

characterized in that the aperture plate arrangement (17) is hereby disposed in the intermediate image plane produced by the microlenses (4).

11. The arrangement according to claim 6,

characterized in that the detection optics (21) comprises a CCD camera.

12. The arrangement according to claim 6,

characterized in that the detection optics (21) has a filter (19).

13. A device for hybridizing the chip with sample material according to one or several of the claims 1 through 5,

characterized by a cup-shaped element (7) for receiving the underside of the chip (1), said cup-shaped element (7) receiving the sample material (10) and said cup-shaped element (7) being adapted to preferably periodically cover the active surface (3) of the elevations (2) in the region of the bottom (8) thereof so that the reading can be configured to be a dynamical process by varying the parameters.

14. The device according to claim 12,

characterized in that the cup-shaped element (7) is tightly closed by the chip (1), an access (9) being provided for producing a negative pressure in the cup-shaped element (7).

15. The device according to claim 13,

characterized in that the bottom (8) is configured to be a membrane.