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Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection

  • US 20040002073A1
  • Filed: 10/15/2002
  • Published: 01/01/2004
  • Est. Priority Date: 10/15/2001
  • Status: Abandoned Application
First Claim
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1. A method of concurrent determination of nucleotide composition at designated polymorphic sites located within one or more target nucleotide sequences, said method comprising the following steps:

  • (a) providing one or more sets of probes, each probe capable of annealing to a subsequence of said one or more target nucleotide sequences located within a range of proximity to a designated polymorphic site;

    (b) contacting the set of probes with said one or more target nucleotide sequences so as to permit formation of hybridization complexes by placing an interrogation site within a probe sequence in direct alignment with the designated polymorphic site;

    (c) for each hybridization complex, determining the presence of a match or a mismatch between the interrogation site and a designated polymorphic site; and

    (d) determining the composition of the designated polymorphic site.

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