Enhanced diffraction-based biosensor devices
First Claim
1. A method of detecting an analyte of interest in a medium with a diffraction based biosensor having at least one additional detection tag mechanism, comprising:
- exposing a detection tag material to the medium, the detection tag material tagging the analyte of interest with a measurable parameter in addition to optical diffraction characteristics of the analyte of interest;
exposing a biosensor to the medium, the biosensor having a substrate member with a receptive material layer applied to at least one side thereof wherein the receptive material is specific for the analyte of interest such that the analyte binds to the receptive material;
exposing the biosensor to a light source and detecting the presence of the analyte by detecting a diffraction pattern of the transmitted light or reflected light; and
detecting the emitted measurable parameter of the detection tag material.
2 Assignments
0 Petitions
Accused Products
Abstract
An enhanced diffraction based biosensor system and method are provided for detecting an analyte of interest in a test medium. The system incorporates at least one additional detection tag substance with the analyte of interest, the tag emitting a measurable parameter that is different from optical diffraction characteristics of the analyte. The biosensor may be a “fluoroptical” system wherein the detection tag is a fluorescence emitting substance, including fluorescent-labeled diffraction enhancing elements. The enhanced diffraction biosensor system may determine the presence of analytes in biological fluids both qualitatively and quantitatively.
-
Citations
34 Claims
-
1. A method of detecting an analyte of interest in a medium with a diffraction based biosensor having at least one additional detection tag mechanism, comprising:
-
exposing a detection tag material to the medium, the detection tag material tagging the analyte of interest with a measurable parameter in addition to optical diffraction characteristics of the analyte of interest;
exposing a biosensor to the medium, the biosensor having a substrate member with a receptive material layer applied to at least one side thereof wherein the receptive material is specific for the analyte of interest such that the analyte binds to the receptive material;
exposing the biosensor to a light source and detecting the presence of the analyte by detecting a diffraction pattern of the transmitted light or reflected light; and
detecting the emitted measurable parameter of the detection tag material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. An enhanced diffraction based biosensor system for detecting an analyte of interest in a test medium, said system comprising:
-
a substrate member having a receptive material applied to at least one surface thereof, said receptive material being specific for the analyte of interest;
a detection tag material having a measurable emitted parameter in addition to optical characteristics of the analyte of interest, said detection tag material selected to specifically tag the analyte of interest in the test medium with said measurable parameter; and
wherein upon exposing said substrate member to the test medium, the analyte of interest binds with said receptive material on said substrate member resulting in a detectable diffraction pattern upon subsequent exposure of said substrate member to a light source, and said detection tag material emits said measurable parameter as a second indication that the analyte of interest is present in the test medium. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
-
Specification