Microfluidic closed-end metering systems and methods

• US 20040005247A1
• Filed: 07/03/2002
• Published: 01/08/2004
• Est. Priority Date: 07/03/2002
• Status: Abandoned Application

• Current Assignee: Nanostream, Inc.
• Original Assignee: Nanostream, Inc.
• Inventors: Karp, Christoph D.
• Application Number:
  US10/190,092
• Publication Number:
  US 20040005247A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Microfluidic devices and methods for metering discrete plugs of fluid are provided. The microfluidic devices include an actuating channel, a metering channel and a deformable membrane disposed therebetween. The metering channel is in fluid communication with a fluid source, but is otherwise closed. The pressure in the actuating channel is varied to deform the deformable membrane. The volume of the metering channel varies in proportion with the deformation of the deformable membrane, creating a pressure differential between the metering channel and the fluid source. The pressure differential causes fluid from the fluid source to be drawn into or expelled from the metering channel.

Claims

1. A method for metering at least one microfluidic plug from a larger fluidic volume, the method comprising the steps of:

providing a fluid source;

providing a microfluidic metering channel having an open end and a closed end, the open end being in fluid communication with the fluid source;

providing a first deformable membrane that bounds at least a portion of the metering channel; and

deforming the first deformable membrane to draw a first fluid plug from the fluid source into the metering channel.

2. The method of claim 1, further comprising the step of providing a first actuating channel, at least a portion of the first actuating channel being bounded by the first deformable membrane, wherein the step of deforming the first deformable membrane is performed by altering the pressure within the first actuating channel.

3. The method of claim 2, further comprising the steps of:

providing a second deformable membrane that bounds at least a portion of the metering channel; and

deforming the second deformable membrane to draw a second fluid plug from the fluid source into the metering channel.

4. The method of claim 3, further comprising the step of providing a second actuating channel, at least a portion of the second actuating channel being bounded by the second deformable membrane, wherein the step of deforming the second deformable membrane is performed by altering the pressure within the second actuating channel.

5. The method of claim 1, further comprising the step of providing a first magnetic element associated with the first deformable membrane, wherein the step of deforming the first deformable membrane is performed by applying a first magnetic field to the first magnetic element.

6. The method of claim 3, further comprising the step of providing a second magnetic element associated with second deformable membrane, wherein the step of deforming the second deformable membrane is performed by applying a second magnetic field to the second magnetic element.

7. The method of claim 1, further comprising the step of deforming the deformable membrane to expel the fluid plug from the metering channel.

8. A device for metering at least one microfluidic plug from a larger fluidic volume, the device comprising:

a fluid source;

a metering channel having an open end and a closed end, the open end being in fluid communication with the fluid source; and

a first deformable membrane bounding at least a portion of the metering channel;

wherein the first deformable membrane is adapted to selectively alter the volume of the metering channel to create a first pressure differential between the metering channel and the fluid source.

9. The device of claim 8, further comprising a first actuating channel, at least a portion of the first actuating channel being bounded by the first deformable membrane.

10. The device of claim 8, further comprising a second deformable membrane bounding at least a portion of the metering channel, wherein the second deformable membrane is adapted to selectively alter the volume of the metering channel to create a second pressure differential between the metering channel and the fluid source.

11. The device of claim 10, further comprising a second actuating channel, at least a portion of the second actuating channel being bounded by the second deformable membrane.

12. The device of claim 10 wherein the first deformable membrane and the second deformable membrane are substantially continuous.

13. The device of claim 8, further comprising a first magnetic element associated with the first deformable membrane.

14. The device of claim 10, further comprising a second magnetic element associated with the second deformable membrane.

15. The device of claim 8, wherein the metering channel includes an analytical region.

16. The device of claim 15 wherein the analytical region is substantially optically transmissive.

17. The device of claim 8 wherein the device is fabricated from a plurality of device layers.

18. The device of claim 17 wherein any of the device layers is a stencil layer.

19. The device of claim 17 wherein any of the device layers is fabricated with a polymer.

20. The device of claim 19 wherein the polymer is substantially clear.

21. The device of claim 19 wherein the polymer is selected from the group consisting of:

polyolefins and vinyl-based (alkene-based) polymers.

22. The device of claim 17 wherein any of the device layers is fabricated with a self-adhesive tape material.

23. The device of claim 8 wherein the fluid source is a microfluidic channel.

24. The device of claim 8 wherein the fluid source is a macrofluidic channel.

25. The device of claim 8 wherein the fluid source contains a substantially continuous flow of a liquid.

26. The device of claim 8 wherein first deformable membrane is elastically deformable.

27. A device for metering a plurality of microfluidic plugs from a larger fluidic volume, the device comprising:

a fluid source;

a plurality of metering channels, each having an open end and a closed end, the open end of each metering channel of the plurality of metering channels being in fluid communication with the fluid source; and

a first deformable membrane bounding at least a portion of each metering channel of the plurality of metering channels;

wherein the first deformable membrane is adapted to selectively alter the volume of each metering channel of the plurality of metering channels to create a first pressure differential between each metering channel of the plurality of metering channels and the fluid source.