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Methods and compositions for detection and quantitation of nucleic acid analytes

  • US 20040009506A1
  • Filed: 03/28/2003
  • Published: 01/15/2004
  • Est. Priority Date: 03/29/2002
  • Status: Abandoned Application
First Claim
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1. A method for detecting or quantitating a nucleic acid analyte in a sample, said method comprising:

  • (A) contacting said sample with an analyte-binding oligonucleotide, a labeled oligonucleotide, a capture polymer and a linear stem oligonucleotide under conditions whereby a complex is formed comprising the analyte, analyte-binding oligonucleotide, labeled oligonucleotide, capture polymer and linear stem oligonucleotide, wherein;

    (i) the analyte-binding oligonucleotide comprises (a) a sequence that is hybridizable to the analyte and (b) a sequence that is hybridizable to the stem oligonucleotide;

    (ii) the linear stem oligonucleotide comprises (a) a sequence that is hybridizable to the analyte-binding oligonucleotide and (b) a sequence that is directly or indirectly hybridizable to the labeled oligonucleotide;

    (iii) the labeled oligonucleotide comprises (a) a sequence that is directly or indirectly hybridizable to the stem oligonucleotide and (b) a label capable of directly or indirectly generating a detectable signal;

    (iv) the capture polymer comprises a nucleic acid sequence that is directly or indirectly hybridizable to the analyte;

    (B) detecting or quantitating the complex of step (A);

    whereby detection or quantitation of the complex of step (A) is indicative of presence or quantity of the nucleic acid analyte in the sample.

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