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Assembly for label-free detection of hybridized nucleic targets

  • US 20040009514A1
  • Filed: 05/05/2003
  • Published: 01/15/2004
  • Est. Priority Date: 02/28/2000
  • Status: Abandoned Application
First Claim
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1. An assembly for performing an assay, the assembly comprises:

  • a) an article with a solid support upon which is immobilized a number of first probes, each of said first probes having a second probe attached thereto, forming a duplex, such that each first probe includes a first nucleic acid sequence to which an emitter element is attached, and each second probe includes a second nucleic acid sequence, which is a complementary sequence to said first nucleic acid sequence and containing at least one artificial mismatch site, and to which a quenching element is attached; and

    b) a reagent solution comprising an unlabeled sample nucleic acid target, which has a nucleic sequence that is perfectly complementary to said first-probe nucleic acid sequence and is adapted to displace said second probe from said first probe in competitive hybridization, whereby said emitter element increases in signal intensity.

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