Methods of treatment of periodontal disease
First Claim
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1. An osteogenic protein comprising an amino acid sequence selected from the group consisting of:
- (a) amino acids #32-#129 of FIG. 1 (SEQ ID NO;
2);
(b) amino acids #299-#396 of FIG. 2 (SEQ ID NO;
4); and
(c) amino acids #311-#408 of FIG. 3 (SEQ ID NO;
6).
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Abstract
Purified BMP-2 and BMP-4 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
101 Citations
23 Claims
-
1. An osteogenic protein comprising an amino acid sequence selected from the group consisting of:
-
(a) amino acids #32-#129 of FIG. 1 (SEQ ID NO;
2);
(b) amino acids #299-#396 of FIG. 2 (SEQ ID NO;
4); and
(c) amino acids #311-#408 of FIG. 3 (SEQ ID NO;
6). - View Dependent Claims (9)
-
-
2. An osteogenic protein selected from the group consisting of:
-
(a) a purified BMP-2 protein produced by the steps of (i) culturing a cell transformed with (a) a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 as shown in FIG. 2 (SEQ ID NO;
3); and
(b) sequences which hybridize to sequence (a) under stringent conditions and encode a protein having cartilage and/or bone induction activity (ii) recovering and purifying from said culture medium a protein comprising the 97 amino acid sequence from amino acid #299 to amino acid #396 as shown in FIG. 2 (SEQ ID NO;
4); and
(b) a purified BMP-4 protein produced by the steps of (i) culturing a cell transformed with (a) a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 as shown in FIG. 3 (SEQ ID NO;
5); and
(b) sequences which hybridize to sequence (a) under stringent conditions and encode a protein having cartilage and/or bone induction activity;
(ii) recovering from said culture medium a protein comprising the 97 amino acid sequence from amino acid #311 to amino acid #408 as shown in FIG. 3 (SEQ ID NO;
6).
-
- 3. A BMP-2 protein comprising amino acids #283-#396 of FIG. 2 (SEQ ID NO:
-
5. A purified BMP-2 protein produced by the steps of
(a) culturing a cell transformed with a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 as shown in FIG. 2 (SEQ ID NO: - 3); and
(b) recovering and purifying from said culture medium a protein comprising the 97 amino acid sequence from amino acid #299 to amino acid #396 as shown in FIG. 2 (SEQ ID NO;
4).
- 3); and
- 6. A BMP-4 protein comprising amino acids #293-#408 of FIG. 3 (SEQ ID NO:
-
8. A purified BMP-4 protein produced by the steps of
(a) culturing a cell transformed with a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 as shown in FIG. 3 (SEQ ID NO: - 5); and
(b) recovering form said culture medium a protein comprising the 97 amino acid sequence from amino acid #311 to amino acid #408 as shown in FIG. 3 (SEQ ID NO;
6).
- 5); and
-
10. A pharmaceutical composition comprising an effective amount of a BMP-2 protein in admixture with a pharmaceutically acceptable vehicle.
- 11. A pharmaceutical composition comprising an effective amount of a BMP-4 protein in admixture with a pharmaceutically acceptable vehicle.
- 12. A composition for bone and/or cartilage formation comprising an effective amount of a BMP-2 protein in a pharmaceutically acceptable vehicle.
- 13. A composition for bone and/or cartilage formation comprising an effective amount of a BMP-4 protein in a pharmaceutically acceptable vehicle.
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20. A pharmaceutical composition for wound healing and tissue repair said composition comprising an effective amount of a BMP-2 protein in a pharmaceutically acceptable vehicle.
- 21. A pharmaceutical composition for wound healing and tissue repair said composition comprising an effective amount of a BMP-4 protein in a pharmaceutically acceptable vehicle.
Specification