Methods of treatment of periodontal disease
First Claim
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1. An osteogenic protein comprising an amino acid sequence selected from the group consisting of:
- (a) amino acids #32-#129 of FIG. 1 (SEQ ID NO;
2);
(b) amino acids #299-#396 of FIG. 2 (SEQ ID NO;
4); and
(c) amino acids #311-#408 of FIG. 3 (SEQ ID NO;
6).
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Abstract
Purified BMP-2 and BMP-4 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
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Citations
23 Claims
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1. An osteogenic protein comprising an amino acid sequence selected from the group consisting of:
-
(a) amino acids #32-#129 of FIG. 1 (SEQ ID NO;
2);
(b) amino acids #299-#396 of FIG. 2 (SEQ ID NO;
4); and
(c) amino acids #311-#408 of FIG. 3 (SEQ ID NO;
6). - View Dependent Claims (9)
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2. An osteogenic protein selected from the group consisting of:
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(a) a purified BMP-2 protein produced by the steps of (i) culturing a cell transformed with (a) a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 as shown in FIG. 2 (SEQ ID NO;
3); and
(b) sequences which hybridize to sequence (a) under stringent conditions and encode a protein having cartilage and/or bone induction activity (ii) recovering and purifying from said culture medium a protein comprising the 97 amino acid sequence from amino acid #299 to amino acid #396 as shown in FIG. 2 (SEQ ID NO;
4); and
(b) a purified BMP-4 protein produced by the steps of (i) culturing a cell transformed with (a) a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 as shown in FIG. 3 (SEQ ID NO;
5); and
(b) sequences which hybridize to sequence (a) under stringent conditions and encode a protein having cartilage and/or bone induction activity;
(ii) recovering from said culture medium a protein comprising the 97 amino acid sequence from amino acid #311 to amino acid #408 as shown in FIG. 3 (SEQ ID NO;
6).
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- 3. A BMP-2 protein comprising amino acids #283-#396 of FIG. 2 (SEQ ID NO:
-
5. A purified BMP-2 protein produced by the steps of
(a) culturing a cell transformed with a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 as shown in FIG. 2 (SEQ ID NO: - 3); and
(b) recovering and purifying from said culture medium a protein comprising the 97 amino acid sequence from amino acid #299 to amino acid #396 as shown in FIG. 2 (SEQ ID NO;
4).
- 3); and
- 6. A BMP-4 protein comprising amino acids #293-#408 of FIG. 3 (SEQ ID NO:
-
8. A purified BMP-4 protein produced by the steps of
(a) culturing a cell transformed with a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 as shown in FIG. 3 (SEQ ID NO: - 5); and
(b) recovering form said culture medium a protein comprising the 97 amino acid sequence from amino acid #311 to amino acid #408 as shown in FIG. 3 (SEQ ID NO;
6).
- 5); and
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10. A pharmaceutical composition comprising an effective amount of a BMP-2 protein in admixture with a pharmaceutically acceptable vehicle.
- 11. A pharmaceutical composition comprising an effective amount of a BMP-4 protein in admixture with a pharmaceutically acceptable vehicle.
- 12. A composition for bone and/or cartilage formation comprising an effective amount of a BMP-2 protein in a pharmaceutically acceptable vehicle.
- 13. A composition for bone and/or cartilage formation comprising an effective amount of a BMP-4 protein in a pharmaceutically acceptable vehicle.
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20. A pharmaceutical composition for wound healing and tissue repair said composition comprising an effective amount of a BMP-2 protein in a pharmaceutically acceptable vehicle.
- 21. A pharmaceutical composition for wound healing and tissue repair said composition comprising an effective amount of a BMP-4 protein in a pharmaceutically acceptable vehicle.
Specification